当前位置：
X-MOL 学术
›
Microsc. Res. Tech.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Efficacy of the photon-initiated photoacoustic streaming combined with different solutions on Enterococcus faecalis in the root canals.
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-02-16 , DOI: 10.1002/jemt.23455 Cheng Wen 1 , Yuanyuan Kong 1 , Jian Zhao 1 , Yang Li 1 , Miao Yu 1 , Sujuan Zeng 1 , Zhan Shi 2 , Qianzhou Jiang 1
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-02-16 , DOI: 10.1002/jemt.23455 Cheng Wen 1 , Yuanyuan Kong 1 , Jian Zhao 1 , Yang Li 1 , Miao Yu 1 , Sujuan Zeng 1 , Zhan Shi 2 , Qianzhou Jiang 1
Affiliation
|
This study aimed to evaluate the efficacy of different irrigation solutions used in photon‐initiated photoacoustic streaming (PIPS) or conventional needle irrigation (CNI) for eradication of Enterococcus faecalis from artificial root canals. Altogether, 240 artificial root canal samples were included. The models were split and incubated for 2 days to allow formation of E. faecalis biofilm. The models were randomly divided into two groups (n = 120): CNI and laser‐activated irrigation (LAI). Each group was divided into six subgroups according to different irrigation solutions: distilled water, 1% sodium hypochlorite (NaOCl), 2% NaOCl, 5.25% NaOCl, MTAD, and chlorhexidine, respectively. After irrigation, half of the samples (n = 10) were assessed immediately, and the other half of the samples (n = 10) were incubated for 6 hr. Bacterial suspensions were obtained from all samples before and after irrigation, and after incubation, and were quantified adenosine 5′‐triphosphate (ATP) assay kit. The biofilms were examined using fluorescent microscopy and analyzed by Image Pro Plus software. Significant reduction of ATP, average fluorescence density after irrigation, and growth after incubation was obtained in LAI group than in CNI group (p < .05). LAI can improve bacteriostasis effect of 2% NaOCl (p < .05). PIPS improved the antibacterial effect of the 2% NaOCl used in root canal therapy.
中文翻译：
光子引发的光声流与根管内粪肠球菌的不同解决方案相结合的功效。
这项研究旨在评估光子引发的光声流（PIPS）或常规针头灌溉（CNI）中使用的不同冲洗液从人工根管中清除粪肠球菌的功效。总共包括240个人工根管样品。分离模型并温育2天，以形成粪肠球菌生物膜。模型随机分为两组（n = 120）：CNI和激光激活灌溉（LAI）。根据不同的灌溉方式将每组分为六个亚组：分别为蒸馏水，1％次氯酸钠（NaOCl），2％NaOCl，5.25％NaOCl，MTAD和洗必太。灌溉后，一半样品（n立即评估= 10），将另一半样品（n = 10）孵育6小时。在冲洗前后，孵育后从所有样品中获得细菌悬液，并用5'-三磷酸腺苷（ATP）定量试剂盒进行定量。使用荧光显微镜检查生物膜，并通过Image Pro Plus软件进行分析。与CNI组相比，LAI组的ATP，灌溉后的平均荧光密度和孵育后的生长显着降低（p < .05）。LAI可以提高2％NaOCl的抑菌作用（p < .05）。PIPS改善了用于根管治疗的2％NaOCl的抗菌作用。
更新日期：2020-02-16
中文翻译：
光子引发的光声流与根管内粪肠球菌的不同解决方案相结合的功效。
这项研究旨在评估光子引发的光声流（PIPS）或常规针头灌溉（CNI）中使用的不同冲洗液从人工根管中清除粪肠球菌的功效。总共包括240个人工根管样品。分离模型并温育2天，以形成粪肠球菌生物膜。模型随机分为两组（n = 120）：CNI和激光激活灌溉（LAI）。根据不同的灌溉方式将每组分为六个亚组：分别为蒸馏水，1％次氯酸钠（NaOCl），2％NaOCl，5.25％NaOCl，MTAD和洗必太。灌溉后，一半样品（n立即评估= 10），将另一半样品（n = 10）孵育6小时。在冲洗前后，孵育后从所有样品中获得细菌悬液，并用5'-三磷酸腺苷（ATP）定量试剂盒进行定量。使用荧光显微镜检查生物膜，并通过Image Pro Plus软件进行分析。与CNI组相比，LAI组的ATP，灌溉后的平均荧光密度和孵育后的生长显着降低（p < .05）。LAI可以提高2％NaOCl的抑菌作用（p < .05）。PIPS改善了用于根管治疗的2％NaOCl的抗菌作用。
京公网安备 11010802027423号