Effects of two seminal fluid transcripts on post‐mating behavior in the simultaneously hermaphroditic flatworm Macrostomum lignano,Journal of Evolutionary Biology

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Effects of two seminal fluid transcripts on post‐mating behavior in the simultaneously hermaphroditic flatworm Macrostomum lignano
Journal of Evolutionary Biology ( IF 2.1 ) Pub Date : 2020-03-06 , DOI: 10.1111/jeb.13606
Michael Weber ₁ , Bahar Patlar ₁ , Steven A Ramm ₁

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The seminal fluid proteins (SFPs) transferred to mating partners along with sperm often play crucial roles in mediating post‐mating sexual selection. One way in which sperm donors can maximize their own reproductive success is by modifying the partner's (sperm recipient's) post‐copulatory behaviour to prevent or delay re‐mating, thereby decreasing the likelihood or intensity of sperm competition. Here, we adopted a quantitative genetic approach combining gene expression and behavioural data to identify candidates that could mediate such a response in the simultaneously hermaphroditic flatworm Macrostomum lignano. We identified two putative SFPs—Mlig‐pro46 and Mlig‐pro63—linked to both mating frequency and ‘suck’ frequency, a distinctive behaviour, in which, upon ejaculate receipt, the worm places its pharynx over its female genital opening and apparently attempts to remove the received ejaculate. We, therefore, performed a manipulative experiment using RNA interference‐induced knockdown to ask how the loss of Mlig‐pro46 and Mlig‐pro63 expression, singly and in combination, affects mating frequency, partner suck propensity and sperm competitive ability. None of the knockdown treatments impacted strongly on the mating frequency or sperm competitive ability, but knockdown of Mlig‐pro63 resulted in a significantly decreased suck propensity of mating partners. This suggests that Mlig‐pro63 may normally act as a cue in the ejaculate to trigger recipient suck behaviour and—given that other proteins in the ejaculate have the opposite effect—could be one component of an ongoing arms race between donors and recipients over the control of ejaculate fate. However, the adaptive significance of Mlig‐pro46 and Mlig‐pro63 from a donor perspective remains enigmatic.

中文翻译：

两种精液转录物对同时雌雄同体的扁虫Macrostomum lignano交配后行为的影响

与精子一起转移到交配对象的精液蛋白 (SFP) 通常在介导交配后的性选择中起着至关重要的作用。精子捐赠者可以最大限度地提高自己的生殖成功的一种方法是通过改变伴侣（精子接受者）的交配后行为来防止或延迟重新交配，从而降低精子竞争的可能性或强度。在这里，我们采用了一种结合基因表达和行为数据的定量遗传方法来确定可以在同时雌雄同体的扁虫 Macrostomum lignano 中调节这种反应的候选物。我们确定了两个假定的 SFP——Mlig-pro46 和 Mlig-pro63——与交配频率和“吸吮”频率相关，这是一种独特的行为，其中，在收到射精时，蠕虫将其咽部放在其女性生殖器开口上，显然试图取出接收到的精液。因此，我们使用 RNA 干扰诱导的敲低进行了一项操作性实验，以了解 Mlig-pro46 和 Mlig-pro63 表达的缺失如何单独和组合影响交配频率、伴侣吸吮倾向和精子竞争能力。没有一种敲低处理对交配频率或精子竞争力产生强烈影响，但 Mlig-pro63 的敲低导致交配伙伴的吸吮倾向显着降低。这表明 Mlig-pro63 通常可以作为射精中的一个线索来触发接受者的吸吮行为，而且——鉴于射精中的其他蛋白质具有相反的效果——可能是供者和接受者之间正在进行的军备竞赛的一个组成部分。射精的命运。然而，从供体的角度来看，Mlig-pro46 和 Mlig-pro63 的适应性意义仍然是个谜。

更新日期：2020-03-06

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