A novel fluorescent aptasensor based on the G-quadruplex induced fluorescent quenching of psoralen and the competitive interactions between 4′-aminomethyl-4,5′,8-trimethylpsoralen (AMT), adenosine triphosphate (ATP) and G-rich DNA functionalized split ATP aptamer was proposed. The binding of ATP to the G-rich DNA functionalized split aptamer induced a significant enhancement in fluorescence emission intensity while undergoing excitation at 340 nm. Under the optimal conditions, the developed aptasensor showed high selectivity and good accuracy for detecting ATP. The practicality of the proposed aptasensor has been confirmed by successfully analyzing ATP in spiked human blood serum samples with satisfactory results. As far as we know, this is the first time that the intrinsic quenching ability of G-quadruplex was applied to simply construct a fluorescence turn-on and label-free aptasensor. On account of the superiority of the simplicity of the design strategy, more work is expected in the future to develop a variety of novel sensors for other important analytes using the quenching capability of G-quadruplex through reasonable designs.

一种新型荧光适体传感器，基于 G 四链体诱导的补骨脂素荧光猝灭以及 4'-氨基甲基-4,5',8-三甲基补骨脂素 (AMT)、三磷酸腺苷 (ATP) 和富含 G 的 DNA 功能化裂解 ATP 之间的竞争性相互作用适配体被提出。ATP 与富含 G 的 DNA 功能化分裂适体的结合在 340 nm 激发时引起荧光发射强度显着增强。在最佳条件下，所开发的适配体传感器对ATP检测表现出高选择性和良好的准确性。通过成功分析加标人血清样品中的 ATP，并取得令人满意的结果，证实了所提出的适体传感器的实用性。据我们所知，这是首次利用G-四联体的固有猝灭能力来简单构建荧光开启且无标记的适体传感器。由于设计策略简单的优势，未来有望开展更多工作，通过合理的设计，利用G-四联体的猝灭能力，为其他重要分析物开发各种新型传感器。