Aedes aegypti is the primary vector of several arboviruses that impact human health including the dengue, Zika, and yellow fever viruses. The potential of Ae. aegypti females to transmit viruses is enhanced by mating-induced behavioral and physiological changes that increase female host-seeking behaviors, blood-feeding frequency and longevity. The mating-induced changes are due to female receipt of male seminal fluid proteins (SFPs) during copulation. SFPs also inhibit female re-mating-re-mating incidence is significantly reduced in the initial hours after mating and nearly absent after 24 h. Males, however, are not limited in the number of females they can inseminate and are able to mate with multiple females in succession. As successive mating depletes SFPs, we examined parameters of fertility and re-mating incidence in females after mating with recently mated males. Males of two Ae. aegypti strains (Colombian and Thai) were mated five consecutive times and fecundity, resulting larvae and hatch percentage in each female of the mating sequence was assessed. In both strains, we found that males can mate three times in succession without impacting fertility in their mates. However, significant declines in fecundity, resulting larvae, and hatch percentage were observed after a third mating. Male size influenced female fecundity and fertility as mates of small males showed further reductions compared to mates of big males after mating consecutively. Seven days after the consecutive mating assays, the re-mating rate of females mated fifth in succession was significantly increased (Colombian strain: 33%; Thai strain: 48%) compared to females mated first (0% in both strains). Re-mating incidence was further increased in small, Thai strain males where 82% of fifth mated females re-mated compared to 0% of first mated females. Finally, we show that regardless of male size, mates of experimental males were similarly fertile to mates of control males when mated for a sixth time 48 h after the consecutive mating assays, showing that males recover fertility after 2 d. Our results show that male sexual history influences fertility and re-mating incidence of Ae. aegypti females.

中文翻译：

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男性性史会影响蚊子媒介埃及伊蚊（Diptera：Culicidae）中女性的生育能力和重新交配的发生率。

埃及伊蚊是几种影响人类健康的虫媒病毒的主要载体，其中包括登革热，寨卡病毒和黄热病病毒。Ae的潜力。交配引起的行为和生理变化增加了雌性寻找宿主的行为，补血频率和寿命，从而增强了埃及雌性传播病毒的能力。交配引起的变化是由于在交配过程中雌性收到雄性精液蛋白（SFP）。SFP也抑制雌性交配，在交配后的最初几小时内显着降低了交配的发生率，而在24小时后几乎没有。但是，雄性可以授精的雌性数量并不受限，并且可以连续与多个雌性交配。随着相继耗尽SFP，我们检查了与最近交配过的雄性交配后雌性的生育力和重交发生率的参数。两个Ae的雄性。将埃及埃及菌株（哥伦比亚和泰国）连续五次交配并繁殖，评估交配序列中每只雌性的幼虫和孵化率。在这两种菌株中，我们发现雄性可以连续交配三遍，而不会影响其配偶的生育能力。但是，在第三次交配后，观察到繁殖力显着下降，导致幼虫和孵化率下降。雄性的大小影响雌性繁殖力和繁殖力，因为小雄性的雄性与连续交配后的雄性雄性相比显示出进一步的减少。连续交配测定后的第7天，连续交配第五次交配的雌性交配率显着提高（哥伦比亚菌株：33％；Thai菌株：48％），而先交配的雌性（两种菌株均为0％）。在泰国小型公株中，重新交配的发生率进一步增加，其中五分之一交配雌性的交配率为82％，而第一交配雌性为0％。最后，我们表明，无论雄性大小如何，在连续交配试验后48小时第六次交配时，实验雄性的交配都与对照雄性的交配相似，表明雄性在2 d后恢复了生育能力。我们的研究结果表明，男性性史会影响Ae的生育能力和重婚率。埃及女性。在连续交配试验后48 h进行第六次交配时，实验雄性的交配物与对照雄性的交配物相似地繁殖，这表明雄性在2天后恢复了生育能力。我们的研究结果表明，男性性史会影响Ae的生育能力和重婚率。埃及女性。在连续交配试验后48 h进行第六次交配时，实验雄性的交配物与对照雄性的交配物相似地繁殖，这表明雄性在2天后恢复了生育能力。我们的研究结果表明，男性性史会影响Ae的生育能力和重婚率。埃及女性。