Conservation and expansion of a necrosis-inducing small secreted protein family from host-variable phytopathogens of the Sclerotiniaceae.,Molecular Plant Pathology

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Conservation and expansion of a necrosis-inducing small secreted protein family from host-variable phytopathogens of the Sclerotiniaceae.
Molecular Plant Pathology ( IF 4.8 ) Pub Date : 2020-02-15 , DOI: 10.1111/mpp.12913
Matthew Denton-Giles _{1,

2} , Hannah McCarthy ₂ , Tina Sehrish ₂ , Yasmin Dijkwel ₂ , Carl H Mesarich ₃ , Rosie E Bradshaw ₂ , Murray P Cox ₂ , Paul P Dijkwel ₂

Affiliation

Fungal effector proteins facilitate host‐plant colonization and have generally been characterized as small secreted proteins (SSPs). We classified and functionally tested SSPs from the secretomes of three closely related necrotrophic phytopathogens: Ciborinia camelliae, Botrytis cinerea, and Sclerotinia sclerotiorum. Alignment of predicted SSPs identified a large protein family that share greater than 41% amino acid identity and that have key characteristics of previously described microbe‐associated molecular patterns (MAMPs). Strikingly, 73 of the 75 SSP family members were predicted within the secretome of the host‐specialist C. camelliae with single‐copy homologs identified in the secretomes of the host generalists S. sclerotiorum and B. cinerea. To explore the potential function of this family of SSPs, 10 of the 73 C. camelliae proteins, together with the single‐copy homologs from S. sclerotiorum (SsSSP3) and B. cinerea (BcSSP2), were cloned and expressed as recombinant proteins. Infiltration of SsSSP3 and BcSSP2 into host tissue induced rapid necrosis. In contrast, only one of the 10 tested C. camelliae SSPs was able to induce a limited amount of necrosis. Analysis of chimeric proteins consisting of domains from both a necrosis‐inducing and a non‐necrosis‐inducing SSP demonstrated that the C‐terminus of the S. sclerotiorum SSP is essential for necrosis‐inducing function. Deletion of the BcSSP2 homolog from B. cinerea did not affect growth or pathogenesis. Thus, this research uncovered a family of highly conserved SSPs present in diverse ascomycetes that exhibit contrasting necrosis‐inducing functions.

中文翻译：

菌核菌科宿主可变植物病原体的坏死诱导小分泌蛋白家族的保存和扩展。

真菌效应蛋白促进宿主植物定植，通常被称为小分泌蛋白（SSP）。我们对三种紧密相关的坏死性植物病原体的分泌组进行了分类和功能测试的SSP：Ciborinia camelliae，Botrytis cinerea和Sclerotiania sclerotiorum。预测的SSP的比对确定了一个大的蛋白质家族，该家族具有超过41％的氨基酸同一性，并且具有先前描述的微生物相关分子模式（MAMP）的关键特征。令人惊讶的是，在寄主专科油茶的分泌组中预测了75个SSP家族成员中的73名，并在寄主通才的分泌组中鉴定出单拷贝同源物葡萄球菌和灰葡萄孢。为了探索这个SSP家族的潜在功能，我们克隆了73个茶花茶中的10种蛋白质，以及来自菌核盘菌（SsSSP3）和灰质芽孢杆菌（BcSSP2）的单拷贝同源物，并将其表达为重组蛋白。SsSSP3和BcSSP2渗透到宿主组织中会导致快速坏死。相比之下，测试的10个山茶果SSP中只有1个能够诱导有限量的坏死。对由坏死诱导型和非坏死诱导型SSP的结构域组成的嵌合蛋白的分析表明，核盘菌的C末端SSP对于诱导坏死的功能至关重要。在缺失BcSSP2从同源灰霉病不影响经济增长或发病。因此，这项研究发现了存在于不同的子囊中的一系列高度保守的SSP，它们具有不同的坏死诱导功能。

更新日期：2020-02-15

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