INTRODUCTION Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in various diseases, including cancer. However, little is known about lncRNAs in human brain gliomas. MATERIAL AND METHODS We examined lncRNA profiles from three glioma specimens using lncRNA expression profiling microarrays. Quantitative real-time RT-PCR was used to analyze the differential expression of raw intensities of lncRNA expression in glioma and peritumoral tissues. RESULTS We found 4858 lncRNAs to be differentially expressed between tumor tissue and peritumoral tissue. Of these, 2845 lncRNAs were up-regulated (fold change > 3.0) and 2013 were down-regulated (fold change < 1/3). A total of 4084 messenger RNAs were also differentially expressed, including 2280 up-regulated transcripts (fold change > 3.0) and 1804 that were down-regulated (fold change < 1/3). Consistent with the microarray data, qPCR confirmed differential expression of these 6 lncRNAs (ak125809, ak098473, uc002ehu.1, bc043564, NR_027322, and uc003qmb.2) between tumor and peritumoral tissue. We next established co-expression networks of differentially expressed lncRNAs and mRNAs. Many mRNAs, such as LOC729991, NUDCD1, SHC3, PDGFA, and MDM2, and lncRNAs, such as ENST00000425922, ENST00000455568, uc002ukz.1, ENST00000502715, and NR_027873, have been shown to play important roles in glioma development. Consistent with this, pathway analysis revealed that "GLIOMA" (KEGG Pathway ID: hsa05214) was significantly enriched in tumor tissue. CONCLUSIONS Our data suggest that altered expression of lncRNAs may be a critical determinant of tumorigenesis in glioma patients.

中文翻译：

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长链非编码 RNA (lncRNA) 的异常表达与脑胶质瘤的发展有关。

引言 长链非编码 RNA (lncRNA) 的异常表达与各种疾病有关，包括癌症。然而，人们对人脑胶质瘤中的 lncRNA 知之甚少。材料和方法 我们使用 lncRNA 表达谱微阵列检查了三个胶质瘤标本的 lncRNA 谱。定量实时 RT-PCR 用于分析胶质瘤和瘤周组织中 lncRNA 表达原始强度的差异表达。结果我们发现4858个lncRNA在肿瘤组织和瘤周组织之间存在差异表达。其中，2845 个 lncRNA 被上调（倍数变化 > 3.0），2013 个被下调（倍数变化 < 1/3）。共有 4084 个信使 RNA 也有差异表达，包括 2280 个上调转录物（倍数变化 > 3. 0）和 1804 被下调（倍数变化 < 1/3）。与微阵列数据一致，qPCR 证实了这 6 个 lncRNA（ak125809、ak098473、uc002ehu.1、bc043564、NR_027322 和 uc003qmb.2）在肿瘤和瘤周组织之间的差异表达。我们接下来建立了差异表达的 lncRNA 和 mRNA 的共表达网络。许多 mRNA，如 LOC729991、NUDCD1、SHC3、PDGFA 和 MDM2，以及 lncRNA，如 ENST00000425922、ENST00000455568、uc002ukz.1、ENST00000502715 和 NR_027873，已被证明在胶质瘤发展中发挥重要作用。与此一致，通路分析显示“GLIOMA”（KEGG Pathway ID：hsa05214）在肿瘤组织中显着富集。结论 我们的数据表明，lncRNA 的表达改变可能是胶质瘤患者肿瘤发生的关键决定因素。