BACKGROUND Saccharopolyspora pogona is a prominent industrial strain due to its production of butenyl-spinosyn, a high-quality insecticide against a broad spectrum of insect pests. TetR family proteins are diverse in a tremendous number of microorganisms and some are been researched to have a key role in metabolic regulation. However, specific functions of TetR family proteins in S. pogona are yet to characterize. RESULTS In the present study, the overexpression of the tetR-like gene sp1418 in S. pogona resulted in marked effects on vegetative growth, sporulation, butenyl-spinosyn biosynthesis, and oxidative stress. By using qRT-PCR analysis, mass spectrometry, enzyme activity detection, and sp1418 knockout verification, we showed that most of these effects could be attributed to the overexpression of Sp1418, which modulated enzymes related to the primary metabolism, oxidative stress and secondary metabolism, and thereby resulted in distinct growth characteristics and an unbalanced supply of precursor monomers for butenyl-spinosyn biosynthesis. CONCLUSION This study revealed the function of Sp1418 and enhanced the understanding of the metabolic network in S. pogona, and provided insights into the improvement of secondary metabolite production.

中文翻译：

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TetR家族转录调节剂Sp1418对Saccharopolyspora pogona的全球代谢网络的影响。

背景技术由于其产生丁烯基多杀菌素，丁烯多孢菌素是一种抗广谱害虫的高质量杀虫剂，所以它是一种重要的工业菌株。TetR家族蛋白在多种微生物中是多种多样的，并且已经研究出其中一些在代谢调节中起关键作用。但是，尚未鉴定出S. pogona中TetR家族蛋白的特定功能。结果在本研究中，pogona中tetR样基因sp1418的过表达对营养生长，孢子形成，丁烯基多杀菌素的生物合成和氧化应激产生了显着影响。通过使用qRT-PCR分析，质谱法，酶活性检测和sp1418敲除验证，我们证明了这些作用中的大部分可能归因于Sp1418的过表达，它调节与初级代谢，氧化应激和次级代谢有关的酶，从而导致明显的生长特征和丁烯基-多杀菌素生物合成的前体单体供应不平衡。结论本研究揭示了Sp1418的功能并增强了对S. pogona代谢网络的了解，并为改善次生代谢产物提供了见识。