The extremophile red alga Galdieria sulphuraria was successfully grown immobilized in a twin-layer porous substrate bioreactor (TL-PSBR). A maximal biomass growth rate of 10 g dry weight m-2 day-1 was measured at a photon fluence rate of 200 μmol photons m-2 s-1 with addition of 1% CO2 and a temperature of 34 °C. Under these conditions, a maximal biomass value of 232 g m-2 was attained after 33 days of growth. Phycobilin productivity, however, was highest at a lower photon fluence rate of 100 μmol photons m-2 s-1 and reached a phycobilin value of 14 g m-2, a phycobilin content in the biomass of 63 mg g-1 and a phycobilin growth rate of 0.28 g m-2 day-1 for phycocyanin and 0.23 g m-2 day-1 for allophycocyanin. Addition of CO2 was essential to enhance growth and phycobilin production in G. sulphuraria and further optimization of the cultivation process in the TL-PSBR appears possible using a multi-phase approach, higher growth temperatures and optimization of nutrient supply. It is concluded that autotrophic cultivation of G. sulphuraria in a TL-PSBR is an attractive alternative to suspension cultivation for phycobilin production and applications in bioremediation.

中文翻译：

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固定在双层多孔底物光生物反应器上的Galdieria sulfuraria的生物量和藻胆蛋白产量。

极端嗜热菌红藻Galdieria sulfuraria成功地固定在双层多孔基质生物反应器（TL-PSBR）中生长。在200μmol光子m-2 s-1的光子通量速率下，添加1％CO2和温度为34°C，测得最大生物量生长速率为10 g干重m-2 day-1。在这些条件下，生长33天后，最大生物量值为232 g m-2。然而，藻胆素生产力在较低的光子注量速率为100μmol光子m-2 s-1时最高，达到的藻胆素值为14 g m-2，生物量中的藻胆素含量为63 mg g-1和藻胆素藻蓝蛋白的生长速率为0.28 g m-2 day-1，别甲藻蓝蛋白的生长速率为0.23 g m-2 day-1。添加二氧化碳对于提高G的生长和藻胆素产量至关重要。使用多阶段方法，更高的生长温度和优化养分供应，似乎有可能使硫尿菌和进一步优化TL-PSBR中的培养过程。可以得出结论，在TL-PSBR中自养养硫脲志贺菌是悬浮培养的替代方法，可用于藻胆素生产和生物修复。