Adipose tissue is a reliable source of mesenchymal stromal cells (MSC) for use in regenerative medicine. The aim of this pilot study was to describe the method, and assess the safety and the potential efficacy of transplantation of autologous adipose tissue-derived MSC for the treatment of chronic venous stasis ulcers. Study group consisted of 11 patients (mean age: 66.6 ± 9.5 years) with chronic venous stasis ulcers. Adipose tissue was harvested by tumescent-aspiration method. Stromal cells were separated using a dedicated closed system in a real-time bedside manner. The phenotype of cells was determined immediately after separation. Cell concentrate was implanted subcutaneously around the wound and the wound bed. All ulcers were assessed planimetrically before autotransplantation and every two weeks during the six-month follow-up. During the study all patients received standard local and general treatment. The preparation contained an average of 5.6 × 106 ± 4 × 106 cells per milliliter. The phenotype of 65-82% of transplanted cells expressed MSC markers: CD73+ CD90+ and CD34+. An improvement was observed in 75% of ulcers. The data showed highly significant negative correlation (p < 0.0001) between wound size and wound closure degree. There was no correlation of ulcer healing with other parameters evaluated, including age of the patients. No serious side effects were observed. Autotransplantation of adipose tissue stromal cells may be a safe and promising treatment method for chronic venous ulcers.

中文翻译：

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自体脂肪组织间质基质细胞的自体移植在静脉淤积性溃疡的治疗中。

脂肪组织是用于再生医学的间充质基质细胞（MSC）的可靠来源。这项初步研究的目的是描述该方法，并评估自体脂肪组织来源的MSC移植治疗慢性静脉淤积性溃疡的安全性和潜在疗效。研究组包括11例慢性静脉淤滞性溃疡患者（平均年龄：66.6±9.5岁）。通过肿胀抽吸法收获脂肪组织。使用专用的封闭系统以实时床旁方式分离基质细胞。分离后立即确定细胞的表型。将细胞浓缩物皮下植入伤口和伤口床周围。在自体移植之前和六个月的随访期间每两周对所有溃疡进行平面测量。在研究期间，所有患者均接受标准的局部和一般治疗。该制剂每毫升平均含有5.6×106±4×106个细胞。65-82％的移植细胞表型表达MSC标记：CD73 + CD90 +和CD34 +。观察到75％的溃疡有所改善。数据显示伤口大小和伤口闭合程度之间高度显着负相关（p <0.0001）。溃疡愈合与评估的其他参数（包括患者年龄）没有相关性。没有观察到严重的副作用。脂肪组织基质细胞的自体移植对于慢性静脉溃疡可能是一种安全且有希望的治疗方法。65-82％的移植细胞表型表达MSC标记：CD73 + CD90 +和CD34 +。观察到75％的溃疡有所改善。数据显示伤口大小和伤口闭合程度之间高度显着负相关（p <0.0001）。溃疡愈合与评估的其他参数（包括患者年龄）没有相关性。没有观察到严重的副作用。脂肪组织基质细胞的自体移植可能是一种安全且有前途的治疗慢性静脉溃疡的方法。65-82％的移植细胞表型表达MSC标记：CD73 + CD90 +和CD34 +。观察到75％的溃疡有所改善。数据显示伤口大小和伤口闭合程度之间高度显着负相关（p <0.0001）。溃疡愈合与评估的其他参数（包括患者年龄）没有相关性。没有观察到严重的副作用。脂肪组织基质细胞的自体移植可能是一种安全且有前途的治疗慢性静脉溃疡的方法。没有观察到严重的副作用。脂肪组织基质细胞的自体移植可能是一种安全且有前途的治疗慢性静脉溃疡的方法。没有观察到严重的副作用。脂肪组织基质细胞的自体移植可能是一种安全且有前途的治疗慢性静脉溃疡的方法。