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The role of the VosA-repressed dnjA gene in development and metabolism in Aspergillus species.
Current Genetics ( IF 1.8 ) Pub Date : 2020-02-14 , DOI: 10.1007/s00294-020-01058-y
Ye-Eun Son 1 , He-Jin Cho 1 , Wanping Chen 2 , Sung-Hun Son 1 , Mi-Kyung Lee 3 , Jae-Hyuk Yu 4, 5 , Hee-Soo Park 1
Affiliation  

The DnaJ family of proteins (or J-proteins) are molecular chaperones that govern protein folding, degradation, and translocation in many organisms. Although J-proteins play key roles in eukaryotic and prokaryotic biology, the role of J-proteins in Aspergillus species is currently unknown. In this study, we characterized the dnjA gene, which encodes a putative DnaJ protein, in two Aspergillus species: Aspergillus nidulans and Aspergillus flavus. Expression of the dnjA gene is inhibited by the velvet regulator VosA, which plays a pivotal role in spore survival and metabolism in Aspergillus. The deletion of dnjA decreased the number of asexual spores (conidia), produced abnormal conidiophores, and reduced sexual fruiting bodies (cleistothecia) or sclerotia. In addition, the absence of dnjA caused increased sterigmatocystin or aflatoxin production in A. nidulans and A. flavus, respectively. These results suggest that DnjA plays a conserved role in asexual and sexual development and mycotoxin production in Aspergillus species. However, DnjA also plays a species-specific role; AniDnjA but not AflDnjA, affects conidial viability, trehalose contents, and thermal tolerance of conidia. In plant virulence assay, the infection ability of the ΔAfldnjA mutant decreased in the kernels, suggesting that DnjA plays a crucial role in the pathogenicity of A. flavus. Taken together, these results demonstrate that DnjA is multifunctional in Aspergillus species; it is involved in diverse biological processes, including fungal differentiation and secondary metabolism.

中文翻译:

VosA抑制的dnjA基因在曲霉菌种的发育和代谢中的作用。

DnaJ家族的蛋白质(或J蛋白)是分子伴侣,可控制许多生物中的蛋白质折叠,降解和易位。尽管J蛋白在真核生物和原核生物中起关键作用,但目前尚不了解J蛋白在曲霉菌种中的作用。在这项研究中,我们表征了dnjA基因,该基因编码两种曲霉菌中的一种假定的DnaJ蛋白:构巢曲霉和黄曲霉。天鹅绒调节剂VosA抑制dnjA基因的表达,该蛋白在曲霉菌的孢子存活和代谢中起关键作用。dnjA的缺失减少了无性孢子的数量(分生孢子),产生了异常的分生孢子,并减少了性子实体(菌毛)或菌核。此外,dnjA的缺失会分别导致构巢曲霉和黄曲霉的菌丝体毒素或黄曲霉毒素产量增加。这些结果表明,DnjA在曲霉菌属的无性和有性发育以及霉菌毒素的产生中起着保守的作用。但是,DnjA也起特定物种的作用。AniDnjA但不影响AflDnjA,它会影响分生孢子的活力,海藻糖含量和分生孢子的热耐受性。在植物毒力测定中,ΔAfldnjA突变体在籽粒中的感染能力降低,这表明DnjA在黄曲霉的致病性中起关键作用。综上所述,这些结果表明DnjA在曲霉属种中具有多功能性。它参与多种生物过程,包括真菌分化和次级代谢。这些结果表明,DnjA在曲霉菌属的无性和有性发育以及霉菌毒素的产生中起着保守的作用。但是,DnjA也起特定物种的作用。AniDnjA但不影响AflDnjA,它会影响分生孢子的活力,海藻糖含量和分生孢子的热耐受性。在植物毒力测定中,ΔAfldnjA突变体在籽粒中的感染能力降低,这表明DnjA在黄曲霉的致病性中起关键作用。综上所述,这些结果表明DnjA在曲霉属种中具有多功能性。它参与多种生物过程,包括真菌分化和次级代谢。这些结果表明,DnjA在曲霉菌属的无性和有性发育以及霉菌毒素的产生中起着保守的作用。但是,DnjA也起特定物种的作用。AniDnjA但不影响AflDnjA,它会影响分生孢子的活力,海藻糖含量和分生孢子的热耐受性。在植物毒力测定中,ΔAfldnjA突变体在籽粒中的感染能力降低,这表明DnjA在黄曲霉的致病性中起关键作用。综上所述,这些结果表明DnjA在曲霉属种中具有多功能性。它参与多种生物过程,包括真菌分化和次级代谢。影响分生孢子的生存力,海藻糖含量和分生孢子的耐热性。在植物毒力测定中,ΔAfldnjA突变体在籽粒中的感染能力降低,这表明DnjA在黄曲霉的致病性中起关键作用。综上所述,这些结果表明DnjA在曲霉属种中具有多功能性。它参与多种生物过程,包括真菌分化和次级代谢。影响分生孢子的生存力,海藻糖含量和分生孢子的耐热性。在植物毒力测定中,ΔAfldnjA突变体在籽粒中的感染能力降低,这表明DnjA在黄曲霉的致病性中起关键作用。综上所述,这些结果表明DnjA在曲霉属种中具有多功能性。它参与多种生物过程,包括真菌分化和次级代谢。
更新日期:2020-02-14
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