The feasible fabrication of nerve guidance conduits (NGCs) with good biological performance is important for translation in clinics. In this study, poly(d,l‐lactide‐co‐caprolactone) (PLCL) films loaded with various amounts (wt; 5%, 15%, 25%) of methylcobalamin (MeCbl) are prepared, and are further rolled and sutured to obtain MeCbl‐loaded NGCs. The MeCbl can be released in a sustainable manner up to 21 days. The proliferation and elongation of Schwann cells, and the proliferation of Neuro2a cells are enhanced on these MeCbl‐loaded films. The MeCbl‐loaded NGCs are implanted into rats to induce the regeneration of 10 mm amputated sciatic nerve defects, showing the ability to facilitate the recovery of motor and sensory function, and to promote myelination in peripheral nerve regeneration. In particular, the 15% MeCbl‐loaded PLCL conduit exhibits the most satisfactory recovery of sciatic nerves in rats with the largest diameter and thickest myelinated fibers.

中文翻译：

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装载甲钴胺的PLCL导管可促进周围神经再生。

具有良好生物学性能的神经引导导管（NGC）的可行制造对于临床翻译非常重要。在这项研究中，聚（d，升-lactide-合作准备装有各种量（wt; 5％，15％，25％）的甲基钴胺素（MeCbl）的己内酯（PLCL）薄膜，然后进一步压延和缝合以获得负载MeCbl的NGC。MeCbl可以可持续的方式发布长达21天。这些装有MeCbl的薄膜可增强Schwann细胞的增殖和伸长，以及Neuro2a细胞的增殖。装有MeCbl的NGC植入大鼠体内，诱导10 mm截肢坐骨神经缺损的再生，显示出促进运动和感觉功能恢复的能力，并促进周围神经再生的髓鞘形成。特别是，在直径最大，髓鞘纤维最大的大鼠中，含15％MeCbl的PLCL导管表现出最令人满意的坐骨神经恢复。