CRISPR/Cas9, as a new genome-editing tool, offers new approaches to understand and treat diseases, which is being rapidly applied in various areas of biomedical research including sepsis field. The type II prokaryotic CRISPR/Cas system uses a single-guide RNA (sgRNA) to target the Cas9 nuclease to a specific genomic sequence, which is introduced into disease models for functional characterization and for testing of therapeutic strategies. This incredibly precise technology can be used for therapeutic research of gene-related diseases and to program any sequence in a target cell. Most importantly, the multifunctional capacity of this technology allows simultaneous editing of several genes. In this review, we focus on the basic principles, advantages and limitations of CRISPR/Cas9 and the use of the CRISPR/Cas9 system as a powerful tool in sepsis research and as a new strategy for the treatment of sepsis.

中文翻译：

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CRISPR / Cas9技术在脓毒症研究中的应用。

CRISPR / Cas9作为一种新的基因组编辑工具，提供了了解和治疗疾病的新方法，并迅速应用于包括败血症领域在内的生物医学研究的各个领域。II型原核CRISPR / Cas系统使用单向导RNA（sgRNA）将Cas9核酸酶靶向特定的基因组序列，将其引入疾病模型以进行功能表征和治疗策略测试。这项极其精确的技术可用于基因相关疾病的治疗研究，并可对靶细胞中的任何序列进行编程。最重要的是，这项技术的多功能能力允许同时编辑多个基因。在这篇评论中，我们着重于基本原理，