The main aim of this prospective study was to investigate the effect of the concentration of fat-soluble vitamins A, D, E and K in individual follicles on oocyte quality and developmental competence. The analysis was performed on 313 follicular fluid (FF) samples from 50 patients undergoing ovarian stimulation with intracytoplasmic sperm injection. We demonstrated that the mean concentration of individual vitamins in FF correlated with their level in serum (p < 0.0001). The levels of vitamin D in FF were higher than in serum, while the opposite was observed for other analyzed vitamins. We did not observe a correlation between FF vitamin D concentration with fertilization success. However, we observed its association with embryo development status on day 3. Moreover, we showed a statistically significant negative correlation between the mean day 5 embryo score and the concentration of vitamin D in serum (rS = -0.68 p = 0.01) and follicular fluid (rS = -0.71 p = 0.01). Our study showed that FF concentration of vitamin A and E was helpful in the prediction of fertilization success of each individual oocyte. Moreover, vitamin A and E concentrations in FF were associated with status of embryo development on the third day of culture. Vitamin A was also associated with the embryo quality on day 2 and the embryo development status on day 5 after fertilization. In conclusion, a combination of FF vitamin analysis and routine morphological assessment could allow for a more accurate and sensitive method of determining embryonic developmental competence and enable the selection of a better embryo to transfer and perhaps translating into an increased chance of pregnancy.Abbreviations: in vitro fertilization: IVF; anti-Mullerian hormone: AMH; follicular fluid: FF; intracytoplasmic sperm injection: ICSI; top quality: TQ; vitamin D binding globulin level: VDBP; assisted reproductive technology: ART.

中文翻译：

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卵泡脂溶性维生素是卵母细胞能力的标志。

这项前瞻性研究的主要目的是研究单个卵泡中脂溶性维生素A，D，E和K的浓度对卵母细胞质量和发育能力的影响。该分析是对来自50位接受卵巢刺激并胞浆内精子注射的患者的313个卵泡液（FF）样品进行的。我们证明FF中单个维生素的平均浓度与其血清中的水平相关（p <0.0001）。FF中维生素D的水平高于血清，而其他分析的维生素则相反。我们没有观察到FF维生素D浓度与施肥成功之间的相关性。但是，我们在第3天观察到它与胚胎发育状态的关联。此外，我们显示平均第5天胚胎评分与血清（rS = -0.68 p = 0.01）和卵泡液（rS = -0.71 p = 0.01）中维生素D的浓度之间具有统计学意义的负相关。我们的研究表明，维生素A和E的FF浓度有助于预测每个卵母细胞的受精成功率。此外，在培养的第三天，FF中维生素A和E的浓度与胚胎发育的状态有关。受精后第2天，维生素A也与胚胎质量有关，第5天与胚胎发育状况有关。结论，FF维生素分析和常规形态学评估相结合可以为确定胚胎发育能力提供更准确，更灵敏的方法，并能够选择更好的胚胎进行转移，并可能增加怀孕的机会。试管婴儿; 抗穆勒激素：AMH；卵泡液：FF；胞浆内精子注射：ICSI；高品质：TQ；维生素D结合球蛋白水平：VDBP；辅助生殖技术：ART。