RATIONALE Ambient mass spectrometry techniques are much required in forensic chemistry to evaluate evidence with low analytical interference, high confidence, and accuracy. However, traditional methodologies, such as paper spray ionization, have been shown to present low sensitivity in the analysis of illicit drugs from biological matrices. METHODS Fiber spray ionization mass spectrometry (FSI-MS) was developed using a capillary polypropylene (PP) hollow fiber. Seized samples of drugs, i.e. a tablet, blotter paper, hashish, and cocaine powder, were analyzed. Cocaine was quantified from whole urine by dipping the fiber directly into solution. FSI-MS was tested for the analysis of a sample of urine obtained from a drug abuse suspect. RESULTS The FSI(+) analysis showed the detection of different types of synthetic drugs in tablet and blotter paper samples, e.g. amphetamine, cathinones, phenethylamines, and opioids, while pure cocaine and different types of coca alkaloids were identified from cocaine powder with good sensitivity and high mass accuracy. The hashish analysis by FSI(-) revealed signals of cannabinoids, cannabinoid acids, and cannabinoid derivatives, detected mainly as [M - H]- ions or chlorine adducts [M + Cl]- . The quantification of cocaine in whole urine showed good sensitivity and precision with limits of detection and quantification of 5.16 and 17.21 ng/mL, respectively, linearity above 0.999, and relative standard deviation below 2.71%. The evaluation of seized sample of urine showed the detection of cocaine with relative ion intensity greater than 36%, as well as the metabolites benzoylecgonine and cocaethylene with a relative intensity of 1.4% and 6%, respectively. CONCLUSIONS The developed FSI-MS method has the potential to be applied to forensic sample evaluation as well as to determine illicit drugs from biological matrices in toxicological analysis. The use of a capillary PP fiber has advantages as an extractor agent and ionizing substrate, and also the feature of it being dipped directly into the sample, thus preserving the integrity of the sample, which makes this a very promising ambient mass spectrometry method and relevant to forensic chemistry.

中文翻译：

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法医化学中的纤维喷雾电离质谱：滥用药物的筛选和尿液中可卡因的直接测定。

鉴证鉴证法医学中非常需要环境质谱技术来评估具有低分析干扰，高置信度和准确性的证据。但是，传统方法（如纸喷雾电离）在分析生物基质中的违禁药物时显示出较低的灵敏度。方法使用毛细管聚丙烯（PP）中空纤维开发了纤维喷雾电离质谱（FSI-MS）。分析了缉获的药物样品，例如片剂，吸墨纸，大麻油和可卡因粉。通过将纤维直接浸入溶液中，从全尿中定量可卡因。测试了FSI-MS以分析从吸毒嫌疑人那里获得的尿液样品。结果FSI（+）分析表明，可在片剂和吸油纸样品中检测到不同类型的合成药物，例如苯丙胺，卡西酮，苯乙胺和阿片类药物，而从可卡因粉中鉴定出纯可卡因和不同类型的古柯生物碱具有良好的灵敏度。质量精度高。FSI（-）进行的大麻分析显示了大麻素，大麻酸和大麻素衍生物的信号，主要检测为[MH]-离子或氯加合物[M + Cl]-。全尿中可卡因的定量显示了良好的灵敏度和精确度，检出限和定量限分别为5.16和17.21 ng / mL，线性高于0.999，相对标准偏差低于2.71％。尿液样品的评估表明，可卡因的相对离子强度大于36％，以及相对强度分别为1.4％和6％的代谢产物苯甲酰基芽子碱和可口乙烯。结论所开发的FSI-MS方法有可能应用于法医样品评估以及从毒理学分析中确定生物基质中的违禁药物。使用毛细管PP纤维具有作为萃取剂和电离基质的优势，并且具有将其直接浸入样品中的特征，从而保留了样品的完整性，这使其成为非常有前途的环境质谱方法，并且相关法医化学。结论所开发的FSI-MS方法有可能应用于法医样品评估以及从毒理学分析中确定生物基质中的违禁药物。使用毛细管PP纤维具有作为萃取剂和电离基质的优势，并且具有将其直接浸入样品中的特征，从而保留了样品的完整性，这使其成为非常有前途的环境质谱方法，并且相关法医化学。结论所开发的FSI-MS方法有潜力应用于法医样品评估以及从毒理学分析中确定生物基质中的违禁药物。使用毛细管PP纤维具有作为萃取剂和电离基质的优势，并且具有将其直接浸入样品中的特征，从而保留了样品的完整性，这使其成为非常有前途的环境质谱方法，并且相关法医化学。