Molecular characterization of three caspases from Bostrychus sinensis and their transcriptional responses to bacteria and viruses.,Journal of Fish Diseases

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Molecular characterization of three caspases from Bostrychus sinensis and their transcriptional responses to bacteria and viruses.
Journal of Fish Diseases ( IF 2.2 ) Pub Date : 2020-02-13 , DOI: 10.1111/jfd.13140
Yuehan Ding ₁ , Ke Wei ₁ , Xiao Yang ₁ , Fei Jing ₁ , Bin Shen ₁ , Jianshe Zhang ₁

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The caspase family proteins are aspartate-specific cysteine proteases that transmit extracellular signals to cells, ultimately cause apoptosis and therefore play a key role in cellular immunity. In this study, we cloned and characterized three caspases from Chinese black sleeper (Bostrychus sinensis), Bscasp-1, Bscasp-8 and Bscasp-9. Real-time PCR analysis showed that Bscasp-1, Bscasp-8 and Bscasp-9 were universally expressed in all tested tissues of B. sinensis. Expression analyses showed that after poly(I:C) stimulation and bacterial (Vibrio parahaemolyticus) infection, the three caspases were significantly upregulated. After poly(I:C) stimulation, the change of Bscasp-1 expression in the head kidney was the most obvious; peak expression was about 80.78-fold more than that of the control. In addition, the expression of Bscasp-8 and Bscasp-9 in the peripheral blood and liver was 167.99- and 17.98-fold higher than that in the control group, respectively. After V. parahaemolyticus infection, the expression peaks of Bscasp-1 and Bscasp-8 in the peripheral blood and spleen were 85.82-fold and 280.83-fold that of the control. However, the expression of Bscasp-9 in the peripheral blood was upregulated only 8.33-fold higher than that in the control group. These results indicate that Bscasp-1, Bscasp-8 and Bscasp-9 are likely involved in response to viral and bacterial infection.

更新日期：2020-04-13

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