Intravital imaging of brain vasculature through the intact cranium in vivo is based on the evolution of the fluorescence intensity and provides an ability to characterize various physiological processes in the natural context of cellular resolution. The involuntary motions of the examined subjects often limit in vivo non-invasive functional optical imaging. Conventional imaging diagnostic modalities encounter serious difficulties in correction of artificial motions, associated with the rapid structural variations and fast high dynamics of the intensity values in the collected image sequences, when a common reference cannot be provided. In the current report, we introduce an alternative solution based on a time-space Fourier transform method so-called Kappa-Omega. We demonstrate that the proposed approach is effective for image stabilization of fast dynamic image sequences and can be used autonomously without supervision and assignation of a reference image.

中文翻译：

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时空傅里叶κω'滤波器，用于经颅荧光脑成像时的运动伪影补偿。

通过完整的颅骨在体内对脑血管的活体成像基于荧光强度的演变，并提供了在细胞分辨率的自然背景下表征各种生理过程的能力。被检查对象的非自愿运动通常会限制体内非侵入性功能性光学成像。当不能提供共同的参考时，常规的成像诊断方式在校正人造运动时遇到严重的困难，这与收集的图像序列中强度值的快速结构变化和快速高动态相关。在本报告中，我们介绍了一种基于时空傅立叶变换方法（称为Kappa-Omega）的替代解决方案。