Iron overload inhibits self-renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species.,FEBS Open Bio

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Iron overload inhibits self-renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species.
FEBS Open Bio ( IF 2.8 ) Pub Date : 2020-04-07 , DOI: 10.1002/2211-5463.12811
Zhenbo Han _{1,

2} , Zihang Xu _{1,

2} , Lei Chen ₃ , Danyu Ye _{1,

2} , Yang Yu _{1,

2} , Ying Zhang _{1,

2} , Yang Cao _{1,

2} , Bamba Djibril _{1,

2} , Xiaofei Guo _{1,

2} , Xinlu Gao _{1,

2} , Wenwen Zhang _{1,

2} , Meixi Yu _{1,

2} , Shenzhen Liu _{1,

2} , Gege Yan _{1,

2} , Mengyu Jin _{1,

2} , Qi Huang _{1,

2} , Xiuxiu Wang _{1,

2} , Bingjie Hua _{1,

2} , Chao Feng _{1,

2} , Fan Yang _{1,

2} , Wenya Ma _{1,

2} , Yu Liu ₄

Affiliation

Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration‐dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC‐induced cell‐cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self‐renewal in human pluripotent stem cells.

中文翻译：

铁超负荷通过DNA损伤和活性氧的产生抑制人类多能干细胞的自我更新。

铁超载会影响各种细胞类型的细胞周期，但铁超载对人多能干细胞的影响尚未见报道。在这里，我们显示柠檬酸铁铵以浓度依赖的方式显着抑制了人类胚胎干细胞（hESCs）和人类诱导的多能干细胞（hiPSCs）的增殖能力。此外，去铁胺可保护hESC / hiPSC免受FAC诱导的细胞周期停滞。但是，铁超载不会影响hESC / hiPSC中的多能性。此外，用FAC处理hiPSC会导致过量的活性氧生成和DNA损伤。总的来说，我们的发现为铁稳态在维持人类多能干细胞自我更新中的作用提供了新的见解。

更新日期：2020-04-07

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