Significance. Recent Alzheimer's disease (AD) patient studies have focused on retinal analysis, as the retina is the only part of the central nervous system that can be imaged noninvasively by optical methods. However, as this is a relatively new approach, the occurrence and role of retinal pathological features are still debated. Aim. The retina of an APP/PS1 mouse model was investigated using multicontrast optical coherence tomography (OCT) in order to provide a documentation of what was observed in both transgenic and wild-type mice. Approach. Both eyes of 24 APP/PS1 transgenic mice (age: 45 to 104 weeks) and 15 age-matched wild-type littermates were imaged by the custom-built OCT system. At the end of the experiment, retinas and brains were harvested from a subset of the mice (14 transgenic, 7 age-matched control) in order to compare the in vivo results to histological analysis and to quantify the cortical amyloid beta plaque load. Results. The system provided a combination of standard reflectivity data, polarization-sensitive data, and OCT angiograms. Qualitative and quantitative information from the resultant OCT images was extracted on retinal layer thickness and structure, presence of hyper-reflective foci, phase retardation abnormalities, and retinal vasculature. Conclusions. Although multicontrast OCT revealed abnormal structural properties and phase retardation signals in the retina of this APP/PS1 mouse model, the observations were very similar in transgenic and control mice.

中文翻译：

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用多对比度光学相干断层扫描技术对阿尔茨海默氏病小鼠模型进行视网膜分析。

意义。最近的阿尔茨海默氏病（AD）患者研究已经集中在视网膜分析上，因为视网膜是中枢神经系统中唯一可以通过光学方法无创成像的部分。但是，由于这是一种相对较新的方法，因此仍在争论视网膜病理特征的发生和作用。目标。为了提供在转基因和野生型小鼠中观察到的信息的记录，使用多对比度光学相干断层扫描（OCT）研究了APP / PS1小鼠模型的视网膜。方法。通过定制的OCT系统对24只APP / PS1转基因小鼠（年龄：45至104周）和15只年龄匹配的野生型同窝小鼠的两只眼睛进行了成像。在实验结束时，从一部分小鼠中收获了视网膜和大脑（14个转基因小鼠，7个年龄匹配的对照），以便将体内结果与组织学分析进行比较并量化皮质淀粉样蛋白β斑块负荷。结果。该系统提供了标准反射率数据，偏振敏感数据和OCT血管造影照片的组合。从所得的OCT图像中获取有关视网膜层厚度和结构，高反射灶的存在，相位延迟异常和视网膜脉管系统的定性和定量信息。结论。尽管多对比度OCT在该APP / PS1小鼠模型的视网膜中显示出异常的结构特性和相位延迟信号，但在转基因小鼠和对照小鼠中观察到的结果非常相似。该系统提供了标准反射率数据，偏振敏感数据和OCT血管造影照片的组合。从所得的OCT图像中获取有关视网膜层厚度和结构，高反射灶的存在，相位延迟异常和视网膜脉管系统的定性和定量信息。结论。尽管多对比度OCT在该APP / PS1小鼠模型的视网膜中显示出异常的结构特性和相位延迟信号，但在转基因小鼠和对照小鼠中观察到的结果非常相似。该系统提供了标准反射率数据，偏振敏感数据和OCT血管造影照片的组合。从所得的OCT图像中获取有关视网膜层厚度和结构，高反射灶的存在，相位延迟异常和视网膜脉管系统的定性和定量信息。结论。尽管多对比度OCT在该APP / PS1小鼠模型的视网膜中显示出异常的结构特性和相位延迟信号，但在转基因小鼠和对照小鼠中观察到的结果非常相似。