Long non-coding RNAs (lncRNAs) are known to be involved in tumorigenesis. The functions of LINC00511 in gastric cancer are poorly understood. Quantitative RT-PCR was performed to investigate the levels of LINC00511 in gastric cancer tissues and cell lines. CCK-8, flow cytometry, wound-healing and Transwell assays were performed to examine cellular functions. The underlying regulatory mechanisms of LINC00511 in gastric cancer progression were determined using luciferase reporter and RIP assays. LINC00511 levels were significantly higher in gastric cancer tissues and cell lines than in normal samples. The high expression of LINC00511 in gastric cancer patient samples positively correlated with advanced clinical characters and poor prognosis. Depleting LINC00511 reduced tumor cell proliferation, migration and invasion, slowed tumor growth, and accelerated cell apoptosis. Our mechanistic study results indicated that LINC00511 promotes gastric cancer progression in a miR-515-5p-dependent manner. We established that LINC00511 may contribute to the proliferation and invasion of gastric cancer cells by modulating miR-515-5p, indicating that LINC00511 may be a potential molecular target for the development of anti-cancer drugs.

中文翻译：

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LINC00511 通过海绵化 miR-515-5p 在胃癌中促进增殖和侵袭。

已知长链非编码 RNA (lncRNA) 与肿瘤发生有关。LINC00511 在胃癌中的作用知之甚少。进行定量 RT-PCR 以研究 LINC00511 在胃癌组织和细胞系中的水平。进行 CCK-8、流式细胞术、伤口愈合和 Transwell 测定以检查细胞功能。LINC00511 在胃癌进展中的潜在调控机制是使用荧光素酶报告基因和 RIP 分析确定的。胃癌组织和细胞系中的 LINC00511 水平显着高于正常样本。LINC00511在胃癌患者样本中的高表达与晚期临床特征和不良预后呈正相关。消耗 LINC00511 可减少肿瘤细胞增殖、迁移和侵袭，减缓肿瘤生长，并加速细胞凋亡。我们的机制研究结果表明，LINC00511 以 miR-515-5p 依赖性方式促进胃癌进展。我们确定LINC00511可能通过调节miR-515-5p促进胃癌细胞的增殖和侵袭，表明LINC00511可能是开发抗癌药物的潜在分子靶点。