The applications of cell patterning are widespread due to the high-throughput testing and different resolutions offered by these platforms. Cell patterning has aided in deconvoluting in vivo experiments to better characterize cellular mechanisms and increase therapeutic output. Here, we present a technique for engineering an artificial surface via surface chemistry to form large-scale arrays of cells within a microchannel by employing microstamping. By changing the approach in surface chemistry, H1568 cells were patterned hydrodynamically using immunoaffinity, and neutrophils were patterned through self-assembly via chemotaxis. The high patterning efficiencies (93% for hydrodynamic patterning and 68% for self-assembled patterning) and the lack of secondary adhesion demonstrate the reproducibility of the platform. The interaction between H1568 and neutrophils was visualized and quantified to determine the capability of the platform to encourage cell-cell interaction. With the introduction of H1568 cells into the self-assembled patterning platform, a significant hindrance in the neutrophils' ability to swarm was observed, indicating the important roles of inflammatory mediators within the nonsmall cell lung cancer tumor microenvironment.

中文翻译：

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微通道内的表面工程，用于流体动力和自组装细胞图案化。

由于这些平台提供了高通量测试和不同的分辨率，因此细胞图案化的应用非常广泛。细胞模式有助于体内实验的去卷积，以更好地表征细胞机制并增加治疗效果。在这里，我们提出一种通过表面化学工程化人工表面的技术，以通过使用微戳来在微通道内形成细胞的大规模阵列。通过改变表面化学方法，使用免疫亲和力对H1568细胞进行了流体动力学模式化，并通过趋化作用通过自组装对嗜中性粒细胞进行了模式化。图案形成效率高（流体动力学图案形成效率为93％，自组装图案形成效率为68％）以及没有辅助附着力，证明了平台的可重复性。可视化和量化H1568和嗜中性粒细胞之间的相互作用，以确定平台促进细胞与细胞相互作用的能力。将H1568细胞引入自组装模式平台后，观察到嗜中性粒细胞聚集能力的显着障碍，表明炎症介质在非小细胞肺癌肿瘤微环境中的重要作用。