Diabetes is a metabolic disorder that damages many organs. We investigated the effects of reperfusion using lactate Ringer's solution (LR) in a diabetic animal model. Eight-week-old rats were divided into groups: control, hemorrhagic shock induced (HS), diabetes mellitus (DM), DM plus HS (DM + HS) and DM rats that received LR after HS (DM + HS + LR). HS was induced by withdrawing blood from the femoral artery and arterial pressure was maintained at 40 mm Hg for 1 h. Animals were perfused with either withdrawn blood or LR. Rats were sacrificed and hearts were collected from all groups. Histopathological studies were performed using left ventricles and western blotting analysis was performed using protein extracted from the left ventricle. Using the TUNEL assay, we found more apoptotic cells in the DM + HS group compared to the control group, whereas in animals resuscitated with LR, the number of apoptotic cells was reduced. Western blotting showed a significant reduction in apoptotic markers, cyt c, cas 9 and cas 3, and increased survival markers, pPI3K and pAKT, in the DM + HS + LR group. Reperfusion with LR may have therapeutic effects on trauma induced HS by blocking the IGF II R facilitated apoptosis pathway in diabetic rats.

中文翻译：

---

使用乳酸林格氏液再灌注可部分消除糖尿病大鼠失血性休克引起的IGF II受体参与的心脏损害。

糖尿病是一种代谢紊乱，会损害许多器官。我们研究了在糖尿病动物模型中使用乳酸林格氏液（LR）进行再灌注的效果。将八周大的大鼠分为两组：对照组，失血性休克诱导（HS），糖尿病（DM），DM加HS（DM + HS）和在HS后接受LR的DM大鼠（DM + HS + LR）。通过从股动脉中抽血诱导HS，并将动脉压维持在40 mm Hg下1 h。给动物灌注血液或LR。处死大鼠并从所有组收集心脏。使用左心室进行组织病理学研究，并使用从左心室提取的蛋白质进行蛋白质印迹分析。通过TUNEL分析，与对照组相比，DM + HS组中的凋亡细胞更多，而在用LR复苏的动物中，凋亡细胞的数量减少了。Western印迹显示，DM + HS + LR组的凋亡标志物cyt c，cas 9和cas 3显着减少，存活标志物pPI3K和pAKT增加。LR再灌注可通过阻断IGF II R促进的糖尿病大鼠凋亡途径对创伤性HS产生治疗作用。