Abstract

In an aquatic environment, the presence of Bisphenol A (BPA) adversely affects reproduction, biology, behavior, gonads, and early larval development of fish due to being endocrine-disrupting compound. In addition, the detected concentration of BPA in water bodies is reported to be higher than 0.41 μg/L. As an alternative tool, sperm cells are used in toxicological assays for the reliable and practical assessment. For these reasons, we examined the effects of in vitro exposure of BPA on sperm quality of chub Squalius oriantalis and Padanian barbel Barbus plebejus. Spermatozoa were exposed to lower concentrations (0, 0.5, 1.25, 2.5, and 5 µg/L) of BPA for 2 h. The enzymatic activities [glutathione peroxidase (GSH-Px), catalase (CAT), and superoxide dismutase (SOD)] and lipid peroxidation (MDA) were evaluated in spermatozoa. The results demonstrated that BPA exposure significantly decreased activities of SOD and GSH-Px but increased CAT activity and lipid peroxidation (MDA). Compared to control, the percentage and duration of sperm motility significantly decreased. Overall, spermatozoa clearly showed the sensitivity to lower concentrations of BPA.

摘要

在水生环境中，由于是内分泌干扰化合物，双酚 A (BPA) 的存在会对鱼类的繁殖、生物学、行为、性腺和早期幼虫发育产生不利影响。此外，据报道，水体中检测到的 BPA 浓度高于 0.41 μg/L。作为一种替代工具，精子细胞用于毒理学分析，以进行可靠和实用的评估。出于这些原因，我们检查了BPA体外暴露对 chub Squalius oriantalis和 Padanian barbel Barbus plebejus精子质量的影响. 精子暴露在较低浓度（0、0.5、1.25、2.5 和 5 µg/L）的 BPA 中 2 小时。在精子中评估酶活性 [谷胱甘肽过氧化物酶 (GSH-Px)、过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD)] 和脂质过氧化 (MDA)。结果表明，BPA 暴露显着降低了 SOD 和 GSH-Px 的活性，但增加了 CAT 活性和脂质过氧化 (MDA)。与对照组相比，精子活力的百分比和持续时间显着降低。总体而言，精子清楚地显示出对较低浓度 BPA 的敏感性。