AbstractIntroductionCD64 expression increases on neutrophils during bacterial infections. Recently an increase in CD169 expression has been discovered on monocytes during viral infections. Generally, interferons α (IFNsα) and IFNsγ are key drivers of the infectious host immune response. The purpose of this study was to explore if a link exists between these IFNs and both biomarkers.MethodsWhole blood samples from healthy volunteers were stimulated with either IFNs, interleukins, or infectious extracts, to mimic an infectious state. Expressions of CD64 and CD169 were assessed in these samples by multiple flow cytometry methods, over precise kinetics.ResultsThe expression of CD64 was statistically higher in samples stimulated with IFNγ, and CD169 in those stimulated with IFNα (and all other type I IFNs). Surface expressions are directly induced by their respective IFNs via Janus kinase/signal transducer and activator of transduction pathways within 6 to 8 hours of incubation. Mixing both types of IFNs seemed to indicate that they partially inhibit each other.ConclusionsThe induction of CD169 on monocytes and CD164 on neutrophils by type I and type II IFNs confirms the relevance of these markers for assessing between a viral‐ vs bacterial‐oriented immune response.

中文翻译：

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干扰素在全血 CD64 和 CD169 表达中的作用：病毒或细菌免疫反应之间平衡的相关性

摘要介绍在细菌感染期间，中性粒细胞上的 CD64 表达增加。最近发现病毒感染期间单核细胞上 CD169 表达增加。一般来说，干扰素 α (IFNsα) 和 IFNsγ 是感染性宿主免疫反应的关键驱动因素。本研究的目的是探讨这些干扰素与这两种生物标志物之间是否存在联系。方法使用干扰素、白细胞介素或感染性提取物刺激健康志愿者的全血样本，以模拟感染状态。通过多种流式细胞术方法，通过精确的动力学评估这些样品中 CD64 和 CD169 的表达。结果在用 IFNγ 刺激的样品中，CD64 的表达在统计学上较高，而在用 IFNα（以及所有其他 I 型 IFN）刺激的样品中，CD169 的表达较高。在孵育 6 至 8 小时内，表面表达由各自的 IFN 通过 Janus 激酶/信号转导器和转导途径激活剂直接诱导。混合两种类型的干扰素似乎表明它们部分地相互抑制。结论I 型和 II 型 IFN 对单核细胞上的 CD169 和中性粒细胞上的 CD164 的诱导证实了这些标记物在评估病毒与细菌免疫反应之间的相关性。