Studies have shown the cytoskeleton disorganization produced by diabetes and quantified F‐actin fluorescence in the striated muscles of diabetic animals. However, at present, there are no studies that have quantified F‐actin spatial organization (F‐actin‐SO). Through our research, we analyzed the effect of diabetes on F‐actin‐SO in the cardiac and skeletal muscles of a mouse model. The muscle samples were labeled with phalloidin–rhodamine and analyzed with confocal microscopy. The analysis was done in two dimensions using four approaches: quantitation of (a) phalloidin‐occupied areas; (b) number of F‐actin‐unoccupied areas per muscular fiber; (c) F‐actin filament discontinuity; and (d) costamere periodicity. Our results showed that both the cardiac and skeletal muscles of the control mice had more phalloidin‐occupied areas than the diabetic mice. The skeletal muscles had a significantly higher number of F‐actin‐unoccupied areas per muscular fiber and more F‐actin discontinuities. Additionally, using western blot analyses, we showed that those differences were not due to α‐actin protein expression. Finally, we considered the importance of these findings in dysfunctional contraction, disassembly in cell–cell communication, conduction of muscle impulse, and changes in cell nanomechanics. Our results quantitatively demonstrated that diabetes severely affects F‐actin‐SO in striated muscles.

中文翻译：

---

糖尿病引起横纹肌的F-肌动蛋白空间组织的差异。

研究表明，糖尿病动物的横纹肌中由糖尿病产生的细胞骨架紊乱和定量的F-肌动蛋白荧光。但是，目前尚无量化F-肌动蛋白空间组织（F-actin-SO）的研究。通过我们的研究，我们分析了糖尿病对小鼠模型的心肌和骨骼肌中F-肌动蛋白SO的影响。肌肉样品用鬼笔环肽-若丹明标记，并用共聚焦显微镜分析。使用四种方法在二维上进行了分析：（a）鬼笔环肽占据的区域的定量；（b）每条肌纤维中F-肌动蛋白未被占用的区域数量；（c）F-肌动蛋白丝不连续；（d）costamere周期性。我们的结果表明，与糖尿病小鼠相比，对照小鼠的心肌和骨骼肌中的鬼笔环肽占据的区域更多。骨骼肌的每条肌纤维中F-肌动蛋白未被占用的区域明显多，并且F-肌动蛋白的不连续性更多。此外，使用蛋白质印迹分析，我们发现这些差异不是由于α-肌动蛋白的表达所致。最后，我们考虑了这些发现在功能失调性收缩，细胞间通讯分解，肌肉冲动传导以及细胞纳米力学变化中的重要性。我们的结果定量表明糖尿病严重影响横纹肌中的F-肌动蛋白-SO。我们考虑了这些发现在功能障碍性收缩，细胞间通讯分解，肌肉冲动传导以及细胞纳米力学变化中的重要性。我们的结果定量表明糖尿病严重影响横纹肌中的F-肌动蛋白-SO。我们考虑了这些发现在功能障碍性收缩，细胞间通讯分解，肌肉冲动传导以及细胞纳米力学变化中的重要性。我们的结果定量表明，糖尿病严重影响横纹肌中的F-肌动蛋白-SO。