The marine environment is emerging as a biodiversity resource for the discovery of natural molecules or chemical scaffolds with pharmaceutical activity. Marine microbes have a tremendous ability to sense and respond to their surroundings to survive in a stressful environment by producing different molecules. As oxidative stress is directly or indirectly involved in various pathological conditions in humans, we believe that marine-derived antioxidant molecules will have a great prospect as a novel antioxidant molecule. We, in this work, explored the marine microbial resources from the Gulf of Mannar, Bay of Bengal, India. High-throughput screening of antioxidant molecule producing marine microbes has revealed that extract from Kocuria marina CDMP 10, can effectively reduce the DPPH free radical. Methanolic crude extract obtained by the freeze-thawing was fractionated and purified by using activity guided purification with the help of reverse phase HPLC and analysed through UPLC-MS. Chemical analysis, as well as MS-spectra, indicated that marine bacteria K. marina CDMP 10 derived antioxidant fraction contains the short peptides. The antioxidant activity of the three highly hydrophobic peptides, (Ser–Ser–Gln, Phe–Glu, Asp–Ile and Leu–Glu) was confirmed by in vitro as well as a cell-based assay. These small peptide molecules are noncytotoxic and can prevent the human cells from chemical-induced oxidative stress. Ser–Ser–Gln peptide demonstrated a potent free radical scavenging activity in Hepatocellular carcinoma cell lines. This study suggests that these short peptides from K. marina CDMP 10 may serve as a potential pharmaceutical candidate with antioxidant activity.

海洋环境正在成为一种生物多样性资源，用于发现具有药物活性的天然分子或化学支架。海洋微生物具有巨大的感知和响应周围环境的能力，可以通过产生不同的分子在压力环境中生存。由于氧化应激直接或间接地涉及人类的各种病理状况，我们认为海洋来源的抗氧化剂分子作为一种新型抗氧化剂分子将具有广阔的前景。在这项工作中，我们探索了印度孟加拉湾马纳尔湾的海洋微生物资源。高通量筛选抗氧化剂分子产生的海洋微生物表明，从滨海小提琴提取CDMP 10，可有效减少DPPH自由基。将通过冷冻-解冻获得的甲醇粗提取物分级分离，并在反相HPLC的帮助下使用活性导向纯化进行纯化，并通过UPLC-MS进行分析。化学分析以及质谱分析表明，海洋细菌滨海假单胞菌CDMP 10衍生的抗氧化剂级分包含短肽。体外证实了三种高度疏水性肽（Ser–Ser–Gln，Phe–Glu，Asp–Ile和Leu–Glu）的抗氧化活性。以及基于细胞的检测 这些小肽分子无细胞毒性，可以防止人类细胞受到化学诱导的氧化应激。Ser–Ser–Gln肽在肝癌细胞系中表现出有效的自由基清除活性。这项研究表明，来自滨海假单胞菌CDMP 10的这些短肽可能具有潜在的抗氧化活性。