BACKGROUND Metabolites in kiwifruit greatly influence nutritional values, however, the dynamic changes in nutrient composition and the gene expression level of yellow kiwifruit has not been studied so far. To investigate the types and accumulation patterns of metabolites, (LC-ESI-MS)-based metabolomics approach and transcriptome was used to analyze the yellow flesh kiwifruit cultivar 'jinshi 1' collected at different stages of days after full bloom. RESULTS In total, 285 metabolites were identified over the kiwifruit developmental stages. The composition of the metabolites of kiwifruit at different stages of development was different. The contents of organic acids and their derivatives were higher at the initial stage of development and then gradually decreased. The contents of lipids and amino acids fluctuated at different stages of development, but did not change significantly. The transcript profiles throughout yellow kiwifruit development was constructed and analyzed, with a focus on the biosynthesis and metabolism of compounds such as sugars, organic acids and AsA, which are indispensable for the development and formation of quality fruit. The transcript levels of genes involved in sucrose and starch metabolism were consistent with the change in soluble sugar and starch content throughout kiwifruit development. The metabolism of AsA acid was primarily through the L-galactose pathway. CONCLUSION The present metabolome and transcriptome approach identified dynamic changes in 5 types of nutrient metabolite levels, and correlations among such levels, in developing fruit. The results provide information that can be used by metabolic engineers and molecular breeders to improve kiwifruit quality. This article is protected by copyright. All rights reserved.

中文翻译：

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采用代谢组学和转录组学方法分析猕猴桃不同发育阶段的营养成分

背景技术猕猴桃中的代谢物对营养价值影响很大，但目前尚未对黄色猕猴桃营养成分的动态变化和基因表达水平进行研究。为了研究代谢物的类型和积累模式，基于（LC-ESI-MS）的代谢组学方法和转录组被用来分析在盛开后不同时期收集的黄色果肉猕猴桃品种“金石1号”。结果 总共在猕猴桃发育阶段鉴定了 285 种代谢物。猕猴桃在不同发育阶段的代谢物成分不同。有机酸及其衍生物的含量在发展初期较高，然后逐渐下降。脂质和氨基酸的含量在不同发育阶段有所波动，但变化不大。构建和分析了整个黄色猕猴桃发育过程中的转录谱，重点关注糖、有机酸和 AsA 等化合物的生物合成和代谢，这些化合物对于优质水果的发育和形成必不可少。参与蔗糖和淀粉代谢的基因的转录水平与整个猕猴桃发育过程中可溶性糖和淀粉含量的变化一致。AsA 酸的代谢主要通过 L-半乳糖途径。结论 目前的代谢组和转录组方法确定了果实发育过程中 5 种营养代谢物水平的动态变化，以及这些水平之间的相关性。结果为代谢工程师和分子育种者提供了可用于提高猕猴桃质量的信息。本文受版权保护。版权所有。