LncRNA TTN-AS1 acts as sponge for miR-15b-5p to regulate FBXW7 expression in ovarian cancer.,Biofactors

当前位置： X-MOL 学术 › Biofactors › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

LncRNA TTN-AS1 acts as sponge for miR-15b-5p to regulate FBXW7 expression in ovarian cancer.
Biofactors ( IF 5.0 ) Pub Date : 2020-02-12 , DOI: 10.1002/biof.1622
Sheng Miao ₁ , Jia Wang ₁ , Lili Xuan ₁ , Xiaojun Liu ₁

Affiliation

Emerging evidence showed that long noncoding RNA (lncRNA) plays crucial roles in regulating various cancer biological behaviors. Titin‐antisense RNA1 (TTN‐AS1) has been reported to have crucial roles in cancers but its role in ovarian cancer remains unknown. The levels of TTN‐AS1, microNRA‐15b‐5p (miR‐15b‐5p), and F‐box and WD repeat domain containing 7 (FBXW7) in ovarian cancer cells were measured by quantitative reverse‐transcription PCR. Targets for TTN‐AS1 and miR‐15b‐5p were predicted by bioinformatic tools, and validated by luciferase activity reporter assay. Cell proliferation, colony formation, and cell apoptosis were analyzed with cell counting kit‐8 assay, colony formation assay, and flow cytometry. Correlation of TTN‐AS1 and FBXW7 was analyzed at gene expression profiling interactive analysis. TTN‐AS1 was found decreased expression in ovarian cancer tissues and cells. Dual‐luciferase activity validated TTN‐AS1 and FBXW7 shared binding site in miR‐15b‐5p. Functional assays showed TTN‐AS1 overexpression inhibits ovarian cancer cell proliferation, colony formation but promotes apoptosis. Rescue experiments showed that knockdown of FBXW7 could partially counteracted the effects of TTN‐AS1 overexpression on ovarian cancer cell behaviors. Our results indicated that the TTN‐AS1/miR‐15b‐5p/FBXW7 axis identified in this work could help to identify treatment biomarkers for ovarian cancer.

更新日期：2020-02-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11