Background: Interleukin-10 (IL-10) is a pleiotropic cytokine, which has both regulatory and stimulatory effects on different immune cell types. Different studies have reported the importance of IL-10 and Transforming growth factor-beta (TGF-p) in the regulation of B cell class switching the production of immunoglobulin A (IgA); however, the underlying mechanisms remain to be fully elucidated. The objective of this study was to investigate the TGF-p response during B stimulation of human B cells by IL-10. Methods: Pan B cells of healthy donors were negatively purified by a magnetic cell separation technique. B cells were cultured with multimeric CD40 ligand (mCD40L) and IL-10 for two and seven days. After harvesting in specific days, TGF-p receptor II and surface IgA expression was determined by flow cytometry, while IgA and TGF-p secretion was assessed by enzyme-linked immunosorbent assay. Results: B cells endogenously expressed TGF-p receptor II and after 48 hours cultivation with mCD40L or mCD40L plus IL-10, both the expression of this receptor and the production of TGF-p were significantly increased. Notably, TGF-p levels following stimulation with mCD40L and IL-10 were higher than those produced by B cells stimulated with mCD40L alone. Furthermore, at day 7 and following IL-10 stimulation, there was a significant rise in the amount of IgA secretion by class-switched plasma cells, which was higher than stimulation with mCD40L alone. Conclusion: Our findings suggest that IL-10 can modulate TGF-p production and TGF-p receptor expression in mCD40-activated human B lymphocytes.

背景：白介素10（IL-10）是一种多效细胞因子，对不同类型的免疫细胞具有调节和刺激作用。不同的研究报道了 IL-10 和转化生长因子-β (TGF-p) 在调节 B 细胞类别转换免疫球蛋白 A (IgA) 产生中的重要性；然而，其根本机制仍有待充分阐明。本研究的目的是研究 IL-10 刺激人类 B 细胞期间 TGF-β 的反应。方法：通过磁性细胞分离技术对健康供体的 Pan B 细胞进行负纯化。 B 细胞与多聚 CD40 配体 (mCD40L) 和 IL-10 一起培养 2 天和 7 天。在特定天数收获后，通过流式细胞术测定TGF-β受体II和表面IgA表达，同时通过酶联免疫吸附测定评估IgA和TGF-β分泌。结果：B细胞内源表达TGF-β受体II，与mCD40L或mCD40L加IL-10培养48小时后，该受体的表达和TGF-β的产生均显着增加。值得注意的是，用 mCD40L 和 IL-10 刺激后的 TGF-β 水平高于仅用 mCD40L 刺激的 B 细胞产生的水平。此外，在第7天和IL-10刺激后，类别转换浆细胞的IgA分泌量显着增加，高于单独使用mCD40L的刺激。结论：我们的研究结果表明，IL-10 可以调节 mCD40 激活的人 B 淋巴细胞中 TGF-β 的产生和 TGF-β 受体的表达。