The success of implantable devices relies heavily on their interaction with the host cells facilitating the osseointegration process. However, with so many new surface modifications, with subtly varying design parameters, in vitro assays can, with proper interpretation, provide valuable information for understanding cellular behavior. This review brings together pertinent in vitro experimental protocols available to researchers and discusses them in relationship to the development of the osteoblast phenotype during bone repair. Consideration is also paid to the influence of endothelial and macrophage cells that can substantially change osteogenic cell activity and thus can provide added value for predicting the osseointegration potential in vivo. Due to the diverse and heterogeneous nature of cell types available for culture use, this review concludes that there is no “gold standard” series of assays. Rather, we present guidance in the experimental design of in vitro assays to better identify those surfaces with promising osteogenic potential.

中文翻译：

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体外询问植入物表面的成骨潜能：当前分析综述。

可植入设备的成功在很大程度上取决于它们与宿主细胞的相互作用，从而促进了骨整合过程。但是，由于有了许多新的表面修饰，并且设计参数有了细微变化，体外分析可以正确解释为理解细胞行为提供有价值的信息。这篇综述汇集了可供研究人员使用的相关体外实验方案，并讨论了它们与骨修复过程中成骨细胞表型发展的关系。还考虑了内皮细胞和巨噬细胞的影响，这些影响可以显着改变成骨细胞的活性，因此可以为预测体内骨整合的潜力提供附加价值。。由于可用于培养的细胞类型具有多样性和异质性，因此本综述得出结论，没有“黄金标准”系列检测方法。相反，我们在体外测定的实验设计中提供了指导，以更好地鉴定具有成骨潜力的那些表面。