Objective

To observe the seminal plasma proteomic composition in men with spinal cord injury orally treated with probenecid, in order to observe pathways associated with increased sperm motility.

Study design

Prospective study.

Setting

Miami Project to Cure Paralysis – University of Miami/Miller School of Medicine.

Participants

Nine men with spinal cord injury, who agreed to participate in the study.

Intervention

Oral treatment with probenecid – 500 mg per day for one week, then 500 mg twice daily [1000 mg total] per day for three weeks.

Outcome measures

Semen analysis as per WHO 2010 guidelines, and seminal plasma proteomics analysis by LC-MS/MS.

Results

In total, 783 proteins were identified, of which, 17 were decreased, while 6 were increased after treatment. The results suggest a new pathway that could be treated by the decrease of biglycan after probenecid treatment.

Conclusion

Oral treatment with probenecid is able to alter the seminal plasma proteome, in pathways that explain decreased innate immune response.

中文翻译：

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接受口服丙磺舒治疗以改善运动能力的脊髓损伤男性精浆的蛋白质组学分析

客观的

观察口服丙磺舒治疗的脊髓损伤男性精浆蛋白质组学组成，以观察与增加精子活力相关的途径。

学习规划

前瞻性研究。

环境

迈阿密治愈瘫痪项目——迈阿密大学/米勒医学院。

参与者

九名脊髓损伤男性同意参加这项研究。

干涉

丙磺舒口服治疗 – 每天 500 毫克，持续一周，然后每天两次 500 毫克 [总共 1000 毫克]，持续三周。

结果措施

根据 WHO 2010 指南进行精液分析，并通过 LC-MS/MS 进行精浆蛋白质组学分析。

结果

共鉴定出 783 种蛋白质，其中 17 种减少，而 6 种在处理后增加。结果表明，丙磺舒治疗后可通过减少双糖链蛋白聚糖来治疗新途径。

结论

用丙磺舒口服治疗能够改变精浆蛋白质组，在解释先天免疫反应降低的途径中。