Vacuoles are useful materials with antimicrobial and anticancerous properties. Vacuolar proteins can discompose macromolecules from the outside of yeast cells. The objective of this study was to determine the function of a protein transported into a vacuole. Specifically, cytosolic protein aldehyde dehydrogenase 6 (ALD6) was used for the delivery to the vacuole. To transport cytosolic protein to the vacuole in this study, a transfer vector including a signal peptide sequence isolated from vacuolar protein proteinase A was designed. A signal peptide is an amino acid sequence in front of the transported protein. Signal peptides have various delivery pathways according to the kind of signal sequence they contain. They play important roles in transporting proteins to organelles, in cellular mechanisms, and the transfer of protein outside and inside eukaryotes. Thus, we focused on the design of a transfer vector containing a signal peptide sequence isolated from the DNA sequence of proteinase A (PEP4). In addition, this study evaluated the expression level of cytosolic ALD6 after being transported into the yeast vacuole. Our results showed that the developed transfer vector was useful for delivering proteins to vacuole by using signal peptide sequence. Therefore, this transfer vector might be used as a tool to deliver target proteins to organelles of interest in eukaryotes.

中文翻译：

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用蛋白酶A的信号肽标记醛脱氢酶6的液泡靶向

液泡是具有抗菌和抗癌特性的有用材料。液泡蛋白可以分解酵母细胞外部的大分子。这项研究的目的是确定运输到液泡中的蛋白质的功能。具体而言，胞质蛋白醛脱氢酶 6 (ALD6) 用于递送至液泡。为了在本研究中将胞质蛋白转运到液泡，设计了一种包括从液泡蛋白酶 A 中分离的信号肽序列的转移载体。信号肽是转运蛋白前面的氨基酸序列。信号肽根据它们包含的信号序列的种类具有不同的递送途径。它们在将蛋白质运输到细胞器、细胞机制、以及蛋白质在真核生物内外的转移。因此，我们专注于设计包含从蛋白酶 A (PEP4) 的 DNA 序列中分离出的信号肽序列的转移载体。此外，本研究还评估了细胞质 ALD6 被转运到酵母液泡后的表达水平。我们的结果表明，开发的转移载体可用于通过使用信号肽序列将蛋白质递送至液泡。因此，该转移载体可用作将靶蛋白递送至真核生物中感兴趣的细胞器的工具。我们的结果表明，开发的转移载体可用于通过使用信号肽序列将蛋白质递送至液泡。因此，该转移载体可用作将靶蛋白递送至真核生物中感兴趣的细胞器的工具。我们的结果表明，开发的转移载体可用于通过使用信号肽序列将蛋白质递送至液泡。因此，该转移载体可用作将靶蛋白递送至真核生物中感兴趣的细胞器的工具。