We investigated using immunohistochemistry the possible protective effects of ascorbic acid, α-tocopherol and selenium during chemotherapy treatment with cyclophosphamide. Thirty female Wistar rats were divided into five groups of six: group 1, untreated control; group 2, 75 µg/kg cyclophosphamide; group 3, 75 µg/kg cyclophosphamide + 150 µg/kg/day α-tocopherol; group 4, 75 µg/kg cyclophosphamide + 200 µg/kg/day ascorbic acid and group 5, 75 µg/kg cyclophosphamide + 40 ppm/kg/day selenium. Proliferating cell nuclear antigen (PCNA) staining was used to detect cell proliferation and AT₁ was used to evaluate structural damage. Caspase-8, caspase-9 and caspase-3 signal molecules were used to investigate apoptosis. In group 2, epithelium, alveolar macrophages, infiltrated lymphocytes and connective tissue were immunostained moderately to strongly with PCNA. Bronchus, alveolar wall and infiltrated lymphocytes were immunostained moderately to strongly with AT₁ and diffuse strong caspase immunoreactions were observed throughout the lung tissue. AT₁ and caspase immunoreactions in groups 4 and 5 were similar to group 2. In group 3, PCNA immunoreactivity was strong in the bronchiolus epithelium, endothelial cell nuclei and in stacks of infiltrated lymphocyte cell nuclei. In group 3, AT₁ and caspase immunoreactions were identical to group 1. It appears that α-tocopherol inhibits lung tissue damage in rats during chemotherapy.

我们使用免疫组织化学研究了抗坏血酸，α-生育酚和硒在用环磷酰胺进行化疗期间的可能的保护作用。将30只Wistar雌性大鼠分为5组，每组6组：第1组，未治疗的对照组；第1组，未治疗组。第2组，75 µg / kg环磷酰胺；第3组，75 µg / kg环磷酰胺+ 150 µg / kg /天，α-生育酚；第4组为75 µg / kg环磷酰胺+ 200 µg / kg /天抗坏血酸，第5组为75 µg / kg环磷酰胺+ 40 ppm / kg /天硒。增殖细胞核抗原（PCNA）染色用于检测细胞增殖和AT ₁被用来评估结构损伤。Caspase-8，caspase-9和caspase-3信号分子用于研究细胞凋亡。在第2组中，用PCNA对上皮，肺泡巨噬细胞，浸润的淋巴细胞和结缔组织进行中度至强度免疫染色。用AT ₁对支气管，肺泡壁和浸润的淋巴细胞进行中度至强烈免疫染色，并在整个肺组织中观察到弥漫性强的半胱天冬酶免疫反应。第4组和第5组的AT ₁和半胱天冬酶免疫反应与第2组相似。在第3组中，支气管上皮，内皮细胞核以及浸润的淋巴细胞细胞核堆叠中PCNA免疫反应较强。在第3组，AT ₁ 半胱氨酸蛋白酶和半胱天冬酶的免疫反应与第1组相同。看来，α-生育酚可以抑制化学疗法对大鼠肺组织的损害。