• VERNALIZATION2 (VRN2), an angiosperm‐specific subunit of the polycomb repressive complex 2 (PRC2), is an oxygen (O₂)‐regulated target of the PCO branch of the PRT6 N‐degron pathway of ubiquitin‐mediated proteolysis. How this post‐translational regulation coordinates VRN2 activity remains to be fully established.
• Here we use Arabidopsis thaliana ecotypes, mutants and transgenic lines to determine how control of VRN2 stability contributes to its functions during plant development.
• VRN2 localizes to endogenous hypoxic regions in aerial and root tissues. In the shoot apex, VRN2 differentially modulates flowering time dependent on photoperiod, whilst its presence in lateral root primordia and the root apical meristem negatively regulates root system architecture. Ectopic accumulation of VRN2 does not enhance its effects on flowering, but does potentiate its repressive effects on root growth. In late‐flowering vernalization‐dependent ecotypes, VRN2 is only active outside meristems when its proteolysis is inhibited in response to cold exposure, as its function requires concomitant cold‐triggered increases in other PRC2 subunits and cofactors.
• We conclude that the O₂‐sensitive N‐degron of VRN2 has a dual function, confining VRN2 to meristems and primordia, where it has specific developmental roles, whilst also permitting broad accumulation outside of meristems in response to environmental cues, leading to other functions.

中文翻译：

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PRT6 N-degron 途径将 VERNALIZATION 2 限制在内源性缺氧生态位以调节植物发育。

• VERNALIZATION2 (VRN2) 是多梳抑制复合物 2 (PRC2) 的被子植物特异性亚基，是泛素介导的蛋白水解的 PRT6 N-degron 途径的 PCO 分支的氧 (O2) 调节靶标_。这种翻译后调控如何协调 VRN2 活动仍有待完全确定。
• 在这里，我们使用拟南芥生态型、突变体和转基因系来确定 VRN2 稳定性的控制如何有助于其在植物发育过程中的功能。
• VRN2 定位于气生和根组织中的内源性缺氧区域。在茎尖，VRN2 根据光周期差异调节开花时间，而它在侧根原基和根尖分生组织中的存在负调节根系结构。VRN2 的异位积累不会增强其对开花的影响，但会增强其对根系生长的抑制作用。在开花后期依赖春化的生态型中，VRN2 仅在其蛋白水解因冷暴露而受到抑制时才在分生组织外活跃，因为它的功能需要伴随冷触发的其他 PRC2 亚基和辅助因子的增加。
• 我们得出结论，VRN2 的 O ₂敏感 N-degron 具有双重功能，将 VRN2 限制在分生组织和原基中，在那里它具有特定的发育作用，同时还允许在分生组织之外广泛积累以响应环境线索，从而产生其他功能.