S100A7 has been suggested to interact with Ran-binding protein 9. Both proteins are nowadays considered key effectors in immune response. Functional interaction between proteins is ensured by coevolution. The mechanisms of vertebrate coevolution between S100A7 and RanBP9 remain unclear. Several approaches for studying coevolution have been developed. Protein coevolution was inferred by calculating the linear correlation coefficients between inter-protein distance matrices using Mirrortree. We found an overall moderate correlation value (R = 0.53, p < 1e-06). Moreover, owing to the high conservation of RanBP9 protein among vertebrates, we chose to utilize a recent version of Blocks in Sequences (BIS2) algorithm implemented in BIS2Analyzer webserver. A coevolution cluster was identified between the two proteins (p < 8.10e-05). In conclusion, our coevolutionary analysis suggests that amino acid variations may modulate S100A7/RanBP9 interaction with potential pathogenic effects. Such findings could guide further analysis to better elucidate the function of S100A7 and RanBP9 and to design drugs targeting for these molecules in diseases.

中文翻译：

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哺乳动物中的 S100A7/Ran 结合蛋白 9 共同进化。

S100A7 已被建议与 Ran 结合蛋白 9 相互作用。这两种蛋白质现在都被认为是免疫反应的关键效应物。共同进化确保蛋白质之间的功能相互作用。S100A7 和 RanBP9 之间的脊椎动物协同进化机制尚不清楚。已经开发了几种研究协同进化的方法。通过使用 Mirrortree 计算蛋白质间距离矩阵之间的线性相关系数来推断蛋白质共同进化。我们发现总体中度相关值（R = 0.53，p < 1e-06）。此外，由于脊椎动物中 RanBP9 蛋白的高度保守性，我们选择利用在 BIS2Analyzer 网络服务器中实现的最新版本的序列块 (BIS2) 算法。在两种蛋白质之间确定了一个共同进化簇（p < 8.10e-05）。综上所述，我们的共同进化分析表明，氨基酸变异可能会调节 S100A7/RanBP9 与潜在致病作用的相互作用。这些发现可以指导进一步的分析，以更好地阐明 S100A7 和 RanBP9 的功能，并设计针对这些分子在疾病中的药物。