ARFs in plants mediate auxin signaling transduction and regulate growth process. To determine genome-wide characterization of ARFs family in melon (Cucumis melo L.), ARFs were identified via analysis of information within the melon genomic database, and bioinformatic analyses were performed using various types of software. Based on different treatment methods involving dipping with the growth regulator Fengchanji No. 2 and artificial pollination, Jingmi No. 11 melon was used as the test material, and melon plants with unpollinated ovaries served as controls. The expression of ARFs during the early development of melon was analyzed via qRT-PCR. Seventeen genes that encode ARF proteins were identified in the melon genome for the first time. The expression of these ARFs differed in different tissues. The expression levels of CmARF2, CmARF16-like, CmARF18-like2, and CmARF19-like were especially high in melon fruits. The expression of ARFs during the early development of melon fruits differed in response to the different treatments, which suggested that CmARF9, CmARF16-like, CmARF19-like, CmARF19, CmARF1, CmARF2, CmARF3, and CmARF5 may be associated with melon fruit growth during early development. Interestingly, the increase in the transverse diameter of fruits treated with growth regulators was significantly greater than that of fruits resulting from artificial pollination, while the increase in the longitudinal diameter of the fruits resulting from artificial pollination was significantly greater.

中文翻译：

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甜瓜（Cucumis melo L.）果实发育过程中生长素反应因子（ARF）基因家族的全基因组表征和表达分析

植物中的 ARFs 介导生长素信号转导并调节生长过程。为了确定甜瓜 (Cucumis melo L.) 中 ARF 家族的全基因组特征，通过分析甜瓜基因组数据库中的信息来识别 ARF，并使用各种类型的软件进行生物信息学分析。根据生长调节剂丰产机2号浸渍和人工授粉的不同处理方法，以京密11号甜瓜为供试材料，以未授粉子房的甜瓜植株为对照。通过qRT-PCR分析了甜瓜早期发育过程中ARF的表达。首次在甜瓜基因组中鉴定出 17 个编码 ARF 蛋白的基因。这些 ARF 在不同组织中的表达不同。CmARF2、CmARF16-like、CmARF18-like2 和 CmARF19-like 在瓜类果实中含量特别高。甜瓜果实早期发育过程中 ARFs 的表达因处理不同而不同，提示 CmARF9、CmARF16-like、CmARF19-like、CmARF19、CmARF1、CmARF2、CmARF3 和 CmARF5 可能与甜瓜果实发育过程中的生长有关。早期发展。有趣的是，用生长调节剂处理的果实横向直径的增加显着大于人工授粉的果实，而人工授粉导致的果实纵向直径的增加显着更大。这表明 CmARF9、CmARF16-like、CmARF19-like、CmARF19、CmARF1、CmARF2、CmARF3 和 CmARF5 可能与早期发育过程中甜瓜果实的生长有关。有趣的是，用生长调节剂处理的果实横向直径的增加显着大于人工授粉的果实，而人工授粉导致的果实纵向直径的增加显着更大。这表明 CmARF9、CmARF16-like、CmARF19-like、CmARF19、CmARF1、CmARF2、CmARF3 和 CmARF5 可能与早期发育过程中甜瓜果实的生长有关。有趣的是，用生长调节剂处理的果实横向直径的增加显着大于人工授粉的果实，而人工授粉导致的果实纵向直径的增加显着更大。