Here, we monitored the expression of three genes (hsp70, hsp22, and hsf1) involved in heat shock response in Drosophila melanogaster in males and females of different age. Also, we investigated age- and sex-dependent expression of three major genes participating in the production of hydrogen sulfide (H₂S) (cse, cbs, and mst), implicated in stress resistance and aging. In addition to the control strain, we monitored the expression of all of these genes in a cbs knockout strain (cbs−/−) generated using the CRISPR technique. The tested strains differ in the induction capacities of the studied genes. Relative to the control strain, under normal conditions, the cbs−/− strain expresses all of the studied genes more abundantly, especially hsp22. In the control strain, aging leads to a dramatic increase in hsp22 synthesis, whereas in the cbs−/− strain, hsp22 induction is not pronounced. Furthermore, in 30-day-old cbs−/− flies, the constitutive expression of hsp70 and mst is decreased. Surprisingly, in the cbs−/− strain, we detected an upregulation of hsf1 transcription in the 30-day-old females. After heat shock in the control strain, hsp70 and hsp22 induction decreased with age in males and hsp22 decreased in females, while in the cbs−/− strain, a pronounced drop in the induction capacity of both hsp genes was seen in 30-day-old males and females. However, in most cases, the expression levels of hsf1 and H₂S-producing genes do not exhibit pronounced changes depending on sex, age, or heat shock. Flies of control and cbs−/− strain exhibited strong reduction in basal thermotolerance with age. Our data suggest a cross-talk between the two studied ancient and universal adaptive systems.

中文翻译：

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果蝇中两种适应原系统和耐热性的年龄依赖性表达谱。


在这里，我们监测了不同年龄的雄性和雌性果蝇中参与热休克反应的三个基因（ hsp70 、 hsp22和hsf1 ）的表达。此外，我们还研究了参与硫化氢 (H ₂ S) 产生的三个主要基因（ cse 、 cbs和mst ）的年龄和性别依赖性表达，这些基因与应激抵抗和衰老有关。除了对照菌株之外，我们还监测了使用 CRISPR 技术生成的cbs敲除菌株 ( cbs −/−) 中所有这些基因的表达。测试的菌株在所研究的基因的诱导能力方面有所不同。相对于对照菌株，在正常条件下， cbs -/− 菌株更丰富地表达所有研究的基因，尤其是hsp22 。在对照菌株中，衰老导致hsp22合成急剧增加，而在cbs −/− 菌株中， hsp22诱导并不明显。此外，在30天龄的cbs -/− 果蝇中， hsp70和mst的组成型表达降低。令人惊讶的是，在cbs −/− 品系中，我们检测到 30 日龄雌性中hsf1转录上调。对照菌株热休克后，雄性中hsp 70 和hsp22 的诱导能力随着年龄的增长而下降，雌性中hsp22的诱导能力也随之下降，而在cbs −/− 菌株中，两种hsp基因的诱导能力在 30 天内均出现显着下降。 -老年男性和女性。 然而，在大多数情况下， hsf1和H ₂ S产生基因的表达水平不会因性别、年龄或热休克而表现出明显的变化。对照果蝇和cbs -/− 品系的果蝇表现出基础耐热性随着年龄的增长而显着降低。我们的数据表明，所研究的两个古老且普遍的自适应系统之间存在交叉对话。