Objectives Improving biocompatibility of metallic alloy biomaterials has been of great interest to prevent implant associated-diseases, such as stent thrombosis. Herein a simple and efficient procedure was designed to biofunctionalize a biomaterial surface by isolating a SUS316L stainless steel binding peptide. Results After three rounds of phage panning procedure, 12 mer peptide (SBP-A; VQHNTKYSVVIR) was identified as SUS316L-binding peptide. The SBP-A peptide formed a stable bond to a SUS316L modified surface and was not toxic to HUVECs. The SBP-A was then used for anti-ICAM antibody modification on SUS316L to construct a vascular endothelial cell-selective surface. The constructed surface dominantly immobilized vascular endothelial cells to smooth muscle cells, demonstrating that the SBP-A enabled simple immobilization of biomolecules without disturbing their active biological function. Conclusions The SUS316L surface was successfully biofunctionalized using the novel isolated peptide SBP-A, showing its potential as an ideal interface molecule for stent modification. This is the first report of material binding peptide-based optimal surface functionalization to promote endothelialisation. This simple and efficient biofunctionalization procedure is expected to contribute to the development of biocompatible materials.

中文翻译：

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一种用于金属合金的新型生物功能化肽

目的 提高金属合金生物材料的生物相容性对于预防植入物相关疾病（例如支架血栓形成）具有重要意义。本文设计了一种简单有效的程序，通过分离 SUS316L 不锈钢结合肽来生物功能化生物材料表面。结果经过三轮噬菌体淘选程序，12mer肽（SBP-A；VQHNTKYSVVIR）被鉴定为SUS316L结合肽。SBP-A 肽与 SUS316L 修饰表面形成稳定键，对 HUVEC 无毒。然后将SBP-A用于SUS316L上的抗ICAM抗体修饰以构建血管内皮细胞选择性表面。构建的表面主要将血管内皮细胞固定为平滑肌细胞，证明 SBP-A 能够简单地固定生物分子，而不会干扰其活性生物功能。结论 使用新型分离肽 SBP-A 成功地对 SUS316L 表面进行了生物功能化，显示其作为理想的支架修饰界面分子的潜力。这是基于材料结合肽的最佳表面功能化以促进内皮化的第一份报告。这种简单有效的生物功能化程序有望促进生物相容性材料的开发。这是基于材料结合肽的最佳表面功能化以促进内皮化的第一份报告。这种简单有效的生物功能化程序有望促进生物相容性材料的开发。这是基于材料结合肽的最佳表面功能化以促进内皮化的第一份报告。这种简单有效的生物功能化程序有望促进生物相容性材料的开发。