The structure of the MP‐4 protein was previously determined at a resolution of 2.8 Å. Owing to the unavailability of gene‐sequence information at the time, the side‐chain assignment was carried out on the basis of a partial sequence available through Edman degradation, sequence homology to orthologs and electron density. The structure of MP‐4 has now been determined at a higher resolution (2.22 Å) in another space group and all of the structural inferences that were presented in the previous report of the structure were validated. In addition, the present data allowed an improved assignment of side chains and enabled further analysis of the MP‐4 structure, and the accuracy of the assignment was confirmed by the recently available gene sequence. The study reinforces the traditional concept that conservative interpretations of relatively low‐resolution structures remain correct even with the availability of high‐resolution data.

中文翻译：

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来自黧豆的 MP-4 结构，分辨率为 2.22 Å。

MP-4 蛋白的结构先前以 2.8 Å 的分辨率确定。由于当时无法获得基因序列信息，侧链分配是根据通过Edman降解、与直系同源物的序列同源性和电子密度获得的部分序列进行的。MP-4 的结构现已在另一个空间群中以更高分辨率 (2.22 Å) 确定，并且先前结构报告中提出的所有结构推论均得到验证。此外，目前的数据允许改进侧链的分配，并能够进一步分析 MP-4 结构，并且最近可用的基因序列证实了分配的准确性。这项研究强化了传统观念，即即使有高分辨率数据，对相对低分辨率结构的保守解释仍然是正确的。