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Genetic diversity of wild wintering red-crowned crane (Grus japonensis) by microsatellite markers and mitochondrial Cyt B gene sequence in the Yancheng reserve
Animal Biotechnology ( IF 1.7 ) Pub Date : 2020-02-08 , DOI: 10.1080/10495398.2020.1725538
Cheng-He Sun 1 , Hong-Yi Liu 1 , Peng Xu 1 , Chang-Hu Lu 1
Affiliation  

Abstract

The red-crowned crane (Grus japonensis) is one of the most endangered cranes in the world, and its wild population is still declining. To characterize the genetic resources of East Asian migratory populations, we studied the genetic variation in wild red-crowned cranes at the Yancheng reserve. Based on a partial Cyt b gene sequence, 32 wild red-crowned crane samples were screened from 100 feathers with unknown sample information. Twelve haplotypes were detected using 32 wild red-crowned crane samples. Six pairs of published microsatellite primers were selected for genotyping. A total of 47 alleles were obtained, with an average of 7.8 alleles per locus. All microsatellite loci were highly polymorphic; the average polymorphic information content and expected heterozygosity were 0.721 ± 0.080 and 0.768 ± 0.071, respectively. These results show that the East Asian migratory population of wild red-crowned cranes exhibits high polymorphism. These data are useful for informing reintroduction efforts. The study results provide a basis for understanding the population genetic properties of an endangered crane.



中文翻译:

基于微卫星标记和线粒体Cyt B基因序列的盐城保护区野生越冬丹顶鹤遗传多样性

摘要

丹顶鹤(Grus japonensis)是世界上最濒危的鹤类之一,其野生种群数量仍在下降。为了表征东亚迁徙种群的遗传资源,我们研究了盐城保护区野生丹顶鹤的遗传变异。基于部分Cyt b基因序列,从样品信息未知的100根羽毛中筛选出32个野生丹顶鹤样品。使用 32 个野生丹顶鹤样本检测到 12 个单倍型。选择了六对已发表的微卫星引物进行基因分型。共获得 47 个等位基因,平均每个基因座有 7.8 个等位基因。所有微卫星位点都是高度多态的;平均多态信息含量和预期杂合度分别为 0.721±0.080 和 0.768±0.071。这些结果表明,野生丹顶鹤东亚迁徙种群表现出高度的多态性。这些数据有助于为重新引入工作提供信息。研究结果为了解濒危鹤种群遗传特性提供了依据。

更新日期:2020-02-08
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