The previous report shows the minimal promoter (P1) contributes to the Xist RNA activation in cells, while the role of the Xist P1 has not yet been investigated in animal individuals. Here, female Xist P1 knockout rabbits (Xist P1-/-) were generated for the studies. The results showed that there is no significant difference in transmission ratio, Xist and X-linked genes expression, and Xist RNA localization between the female wild type (WT) and Xist P1-/- rabbits, suggesting that P1 is non-essential for Xist expression and XCI in rabbits. Our study has explored the function of Xist P1 in animal level for the first time, and the results provide new ideas for future studies of XCI mechanisms.

中文翻译：

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Xist的最小启动子（P1）对于X染色体灭活不是必需的。

先前的报告显示最小启动子（P1）有助于细胞中Xist RNA的激活，而Xist P1的作用尚未在动物个体中进行研究。在这里，产生雌性Xist P1基因敲除兔（Xist P1-/-）用于研究。结果表明，雌性野生型（WT）和Xist P1-/-兔之间的传动比，Xist和X连锁基因表达以及Xist RNA定位没有显着差异，表明P1对Xist而言不是必需的在兔中的表达和XCI。我们的研究首次探索了Xist P1在动物体内的功能，其结果为XCI机制的未来研究提供了新思路。