Characterizing bacterial communities is of great significance for targeted control of bacteria-induced clogging during geothermal water recharge. Based on a series of laboratory-scale percolation experiments, the variations in bacterial community diversity, composition, and structure were investigated during simulated geothermal water recharge using high-throughput sequencing technology. The Chao, Shannon, and Evenness indexes were used to quantify the richness, diversity, and evenness of the bacterial community, respectively. The results show that the richness of the bacterial community initially increased and then decreased in the sand columns during the experiments of geothermal water recharge, while the changes in bacterial diversity and evenness were not apparent. A variety of bacterial phyla were found, among which Proteobacteria was predominant (88.31%), followed by Actinobacteria, Bacteroidetes, and Firmicutes (4.23%, 3.44%, and 2.49%). For the non-Proteobacterial phyla, Actinobacteria gradually disappeared while Bacteroidetes and Firmicutes were detected during the percolation experiments. This study implies that, despite the variations in the bacterial community, a core group of bacteria persists during geothermal water recharge, and thus a targeted control of bacteria-induced clogging during geothermal water recharge should be feasible.

中文翻译：

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地热水补给引起的生物阻塞过程中细菌群落结构的变化。

表征细菌群落对于在地热水补给过程中靶向控制细菌引起的堵塞具有重要意义。基于一系列实验室规模的渗滤实验，使用高通量测序技术研究了模拟地热水补给过程中细菌群落多样性，组成和结构的变化。Chao，Shannon和Evenness指数分别用于量化细菌群落的丰富度，多样性和均匀度。结果表明，在地热水补给实验中，砂柱中细菌群落的富集度先升高后降低，而细菌多样性和均匀性变化不明显。发现了各种细菌门。其中以变形杆菌为主（88.31％），其次是放线杆菌，拟杆菌和硬毛菌（4.23％，3.44％和2.49％）。对于非变形杆菌门，放线杆菌逐渐消失，而在渗滤实验中检测到了拟杆菌和硬毛菌。这项研究表明，尽管细菌群落有所变化，但在地热水补给过程中仍会保留一个核心细菌群，因此有针对性地控制在地热水补给过程中细菌引起的堵塞是可行的。