BACKGROUND In the early stages of rehabilitation after primary amputation, residual limb soft tissues have not been mechanically conditioned to support load and are vulnerable to damage from prosthetic use. There is limited quantitative knowledge of skin and soft tissue response to prosthetic loading. METHODS An in-vivo protocol was developed to establish suitable measures to assess tissue tolerance during loading representative of early prosthesis use. Ten participants without amputation one participant with trans-tibial amputation were recruited, and pressure applied to their calf in increments from 20 to 60 mmHg. Measurements were recorded at relevant skin sites including interface pressures, transcutaneous oxygen (TCPO2) and carbon dioxide (TCPCO2) tensions and inflammatory biomarkers. FINDINGS At the maximum cuff pressure, mean interface pressures were between 66 and 74 mmHg, associated with decreased TCPO2 values. On the release of pressure, the ischaemic response was reversed. Significant upregulation (p < 0.05) in inflammatory biomarker IL-1α and its antagonist IL-1RA were observed at all sites immediately following loading. INTERPRETATION The protocol was successful in applying representative prosthetic loads to lower limb tissues and monitoring the physiological response, both in terms of tissue ischemia and skin inflammation. Results indicated that the measurement approaches were sensitive to changes in interface conditions, offering a promising approach to monitor tissue status for people with amputation.

中文翻译：

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建立测量阵列以评估代表假体使用的负荷期间的组织耐受性。

背景技术在主要截肢后的康复的早期阶段，残余的肢体软组织尚未经过机械调节以支撑负荷，并且容易受到假肢使用的损害。皮肤和软组织对假体负荷反应的定量知识有限。方法制定了一项体内实验方案，以建立合适的措施来评估负荷期间代表早期假体使用的组织耐受性。招募了10名未截肢的参与者和1名经胫骨截肢的参与者，并以20至60 mmHg的增量对小腿施加压力。在相关的皮肤部位记录测量值，包括界面压力，经皮氧气（TCPO2）和二氧化碳（TCPCO2）张力以及炎性生物标志物。在最大袖带压下，平均界面压力在66至74 mmHg之间，与TCPO2值降低有关。释放压力后，缺血反应逆转。加载后立即在所有位点观察到炎症生物标志物IL-1α及其拮抗剂IL-1RA的显着上调（p <0.05）。解释该协议成功地在下肢组织上施加了代表性的假体负载并监测了组织缺血和皮肤炎症方面的生理反应。结果表明，测量方法对界面状况的变化敏感，为监测截肢患者的组织状况提供了一种有希望的方法。加载后立即在所有位点观察到炎症生物标志物IL-1α及其拮抗剂IL-1RA的显着上调（p <0.05）。解释该协议成功地在下肢组织上施加了代表性的假体负载并监测了组织缺血和皮肤炎症方面的生理反应。结果表明，测量方法对界面状况的变化敏感，为监测截肢患者的组织状况提供了一种有前途的方法。加载后立即在所有位点观察到炎症生物标志物IL-1α及其拮抗剂IL-1RA的显着上调（p <0.05）。解释该协议成功地在下肢组织上施加了代表性的假体负载并监测了组织缺血和皮肤炎症方面的生理反应。结果表明，测量方法对界面状况的变化敏感，为监测截肢患者的组织状况提供了一种有希望的方法。在组织缺血和皮肤炎症方面。结果表明，测量方法对界面状况的变化敏感，为监测截肢患者的组织状况提供了一种有前途的方法。在组织缺血和皮肤炎症方面。结果表明，测量方法对界面状况的变化敏感，为监测截肢患者的组织状况提供了一种有希望的方法。