OBJECTIVE Dentin remineralization is of considerable clinical interest for dentin hypersensitivity and developing biomimetic analogs that can regulate hydroxyapatite (HAp) nucleation and growth remains a challenge. This study aimed to evaluate in vitro the potential for dentin remineralization using the following biomimetic in situ prepared poly(catechols): poly(dopamine), poly(DOPA), poly(caffeic acid) and a synthesized DOPA-peptide possessing collagen and calcium-binding domains (DOPA-Ahx-(Gly)3-(Glu)5). METHODS Dentin samples were immersed in a freshly prepared phosphate-buffered saline (PBS) containing the respective catechol and laccase. After the reaction, they were immersed in calcium and phosphate remineralization solution, which was changed every day for 10 days. Samples of intact and demineralized dentin were used as control groups and kept in deionized water under the same experimental conditions. The remineralized dentin was characterized by scanning electron microscopy (SEM), Micro-energy dispersion X-ray fluorescence spectroscopy (μEDX) and X-ray diffraction (XRD). RESULTS The application of different poly(catechols) and DOPA-peptide promoted crystal nucleation and the formation of HAp, which partially covered both the dentin surface and dentinal tubules walls. SIGNIFICANCE By mimicking the role of charged non-collagenous proteins in vivo, polymers consisting of catechol groups showed the ability to modify demineralized dentin surface properties, promoting mineral formation. The use of poly(catechols) may be encouraged for the development of a therapeutic technique for dentin hypersensitivity.

中文翻译：

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生物启发的儿茶酚化学用于牙本质再矿化：一种治疗牙本质过敏的新方法。

目的牙本质再矿化对牙本质过敏具有相当大的临床意义，开发能够调节羟基磷灰石（HAp）成核和生长的仿生类似物仍然是一个挑战。这项研究的目的是使用以下仿生原位制备的聚儿茶酚在体外评估牙本质再矿化的潜力：聚多巴胺，聚DOPA，聚咖啡酸和合成的具有胶原蛋白和钙的DOPA肽。结合域（DOPA-Ahx-（Gly）3-（Glu）5）。方法将牙本质样品浸入新鲜制备的含有儿茶酚和漆酶的磷酸盐缓冲盐水（PBS）中。反应后，将其浸入钙和磷酸盐再矿化溶液中，每天更换10天。将完整和脱矿质牙本质的样品用作对照组，并在相同的实验条件下保存在去离子水中。通过扫描电子显微镜（SEM），微能分散X射线荧光光谱（μEDX）和X射线衍射（XRD）表征了再矿化的牙本质。结果不同聚儿茶酚和DOPA肽的应用促进了晶体成核和HAp的形成，HAp部分覆盖了牙本质表面和牙本质小管壁。意义通过模仿带电的非胶原蛋白在体内的作用，由儿茶酚基团组成的聚合物显示出改性脱矿牙本质表面特性的能力，从而促进了矿物质的形成。可以鼓励使用聚儿茶酚来发展牙本质过敏的治疗技术。