1. This study was performed to evaluate the effects of dietary supplementation of coenzyme Q10 (CoQ10) on laying rate, body weight, plasma metabolites and some liver gene expression in broiler breeder hens.

2. A total of 128 broiler breeder hens (Arbor Acres Plus, 47 weeks of age) were randomly distributed to four dietary groups supplemented with different levels of CoQ10 (0, 300, 600 or 900 mg/kg diet) with four replicates of eight hens each. During 47–54 weeks of age, laying rate, egg mass and body weight were recorded weekly. To assay plasma biochemical indicators, blood samples were collected at 54 weeks of age. At the end of the experiment, for evaluating the abdominal fat weight, liver weight and expression of the adiponectin and proliferator-activated receptor-α (PPAR-α) genes in the liver, eight hens per treatment were selected, weighed and humanely killed by decapitation.

3. Dietary supplementation of CoQ10 linearly decreased abdominal fat weight, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities by increased levels of CoQ10. The plasma levels of glucose, cholesterol and alkaline phosphatase (ALP) activity were quadratically decreased by increased levels of CoQ10. The best plasma levels of glucose, cholesterol and ALP activity were estimated at 562.5, 633.3 and 517.8 mg CoQ10/kg diet, respectively. Adiponectin and PPARα gene expression exhibited a linear increased by increased levels of CoQ10.

4. In conclusion, addition of CoQ10 to the diet influenced lipid metabolism and expression of the adiponectin and PPAR-α genes, which might be partially due to the improvement in mitochondrial metabolism and energy production. However, further studies are necessary to determine the effects of CoQ10 on these indicators in broiler breeder hens during ageing.

1. 本研究旨在评估日粮添加辅酶 Q10 (CoQ10) 对肉种鸡的产蛋率、体重、血浆代谢物和部分肝脏基因表达的影响。

2. 将总共 128 只肉种鸡（Arbor Acres Plus，47 周龄）随机分配到四个添加不同水平 CoQ10（0、300、600 或 900 mg/kg 日粮）的日粮组中，每组 8 组重复 4 次每只母鸡。在 47-54 周龄期间，每周记录产蛋率、卵量和体重。为了测定血浆生化指标，在 54 周龄时采集血样。在实验结束时，为了评估腹部脂肪重量、肝脏重量和肝脏中脂联素和增殖物激活受体-α ( PPAR-α ) 基因的表达，每个处理选择 8 只母鸡，称重并人道处死斩首。

3. CoQ10 的膳食补充通过增加 CoQ10 水平线性降低腹部脂肪重量、丙氨酸氨基转移酶 (ALT) 和天冬氨酸氨基转移酶 (AST) 活性。葡萄糖、胆固醇和碱性磷酸酶 (ALP) 活性的血浆水平随着 CoQ10 水平的增加而呈二次下降。葡萄糖、胆固醇和 ALP 活性的最佳血浆水平分别估计为 562.5、633.3 和 517.8 mg CoQ10/kg 饮食。脂联素和PPARα基因表达随着 CoQ10 水平的增加呈线性增加。

4. 总之，在饮食中添加辅酶Q10会影响脂质代谢以及脂联素和PPAR-α基因的表达，这可能部分是由于线粒体代谢和能量产生的改善。然而，需要进一步的研究来确定辅酶Q10对肉种鸡老化过程中这些指标的影响。