BACKGROUND Respiratory diseases in pigs are the main health concerns for swine producers. Similar to the diseases in human and other animals, respiratory diseases are primary related to morbidity and are the result of infection with bacteria, viruses, or both. B. bronchiseptica causes serious respiratory diseases in the swine airway track. However, the B. bronchiseptica-specific bacteriophage has diverse advantages such as decreasing antibiotic overuse and possible therapeutic potential against bacteria. OBJECTIVE The objects of this study were to investigate the therapeutic effect of specific B. bronchiseptica bacteriophages and to identify genes related to bacteriophage signaling utilizing RNA microarrays in swine nasal turbinate cells. METHODS Bor-BRP-1 phages were applied 24 h prior to B.bronchiseptica infection (1 × 107 cfu/ml) at several concentrations of bacterial infection. Cells were incubated to detect cytokines and 24 h to detect mucin production. And real-time quantitative PCR was performed to examine related genes expression. To determine the change of total gene expression based on B.bronchiseptica and Bor-BRP-1 treatment, we performed RNA sequencing experiments. RESULTS The results showed that B. bronchiseptica induced increased expression of several inflammatory genes such as IL-1β, IL-6, and Muc1 in a dose-dependent manner. However, Bor-BRP-1 induced reduction of gene expression compared to the B. bronchiseptica induction group. In addition, microarrays detected Bor-BRP-1-altered inflammatory gene expression against B. bronchiseptica, reducing B. bronchiseptica-induced airway inflammation in swine epithelial cells. CONCLUSION These results suggest that the specific bacteriophage has a therapeutic potential to defend against B. bronchiseptica infection by altering inflammatory gene expression profiles.

中文翻译：

---

支气管败血博德特氏菌的特定噬菌体调节支气管败血性博德特氏菌诱导的microRNA表达谱，以减少猪鼻甲细胞的炎症。

背景技术猪的呼吸系统疾病是养猪者的主要健康问题。与人类和其他动物的疾病相似，呼吸道疾病与发病率主要相关，并且是细菌，病毒或两者感染的结果。支气管败血波氏杆菌在猪气道内引起严重的呼吸道疾病。但是，支气管败血杆菌特异的噬菌体具有多种优势，例如减少抗生素的过度使用以及对细菌的潜在治疗潜力。目的本研究的目的是研究特定的支气管败血性博德特氏菌噬菌体的治疗效果，并利用RNA微阵列在猪鼻甲细胞中鉴定与噬菌体信号转导相关的基因。方法在B之前24小时应用Bor-BRP-1噬菌体。在几种细菌感染浓度下，支气管败血病感染（1×107 cfu / ml）。孵育细胞以检测细胞因子，并孵育24小时以检测粘蛋白产生。并进行实时定量PCR检查相关基因的表达。为了确定基于支气管败血杆菌和Bor-BRP-1处理的总基因表达的变化，我们进行了RNA测序实验。结果结果表明，支气管败血性芽孢杆菌诱导几种炎症基因如IL-1β，IL-6和Muc1的表达呈剂量依赖性。但是，与支气管败血性博德特氏菌诱导组相比，Bor-BRP-1诱导的基因表达降低。此外，微阵列检测到了Bor-BRP-1改变了针对支气管败血性博德特氏菌的炎性基因表达，从而减少了猪上皮细胞中由支气管败血性博德特氏菌诱导的气道炎症。