IncF ColV plasmids are important plasmid incompatibility group that are currently restricted to the Enterobacteriaceae. These plasmids carry an important repertoire of virulence-associated genes (VAGs) that contribute to the ability of avian pathogenic Escherichia coli to cause disease in poultry. VAGs found on ColV plasmids have also been linked to urosepsis and meningitis in humans but the mechanisms that elicit these disease conditions are not well understood. Recently we described the sequence of a ColV plasmid pSDJ2009-52F that carried the typical repertoire of VAGs and a complex resistance gene locus flanked by IS26, an insertion element that plays an important role in mobilizing antibiotic resistance genes on plasmids and genomic islands. We recovered complete ColV-like plasmid sequences from public databases that shared >80% sequence identity with pSDJ2009-52F in geographically diverse regions of the world over a 20-year timeframe. Previously we noted that pSDJ2009-52F carries a unique genetic signature in the class 1 integron within the complex resistance locus that was presumably created by the action of IS26. Here we show that most ColV-like plasmids that are closely related to pSDJ2009-52F also carry the same signature. Our studies provide insight into how these signature-bearing plasmids and the mobile genetic elements they carry traffic between E. coli sequence types over large geographic distances.

中文翻译：

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IncF ColV样质粒谱系的分子分析，该谱系携带具有可追踪遗传特征的复杂抗性基因座。

IncF ColV质粒是重要的质粒不相容性组，目前仅限于肠杆菌科。这些质粒带有重要的毒力相关基因（VAG），这些基因有助于禽类致病性大肠杆菌引起家禽疾病。在ColV质粒上发现的VAG也与人尿毒症和脑膜炎有关，但引起这些疾病的机制尚不清楚。最近，我们描述了ColV质粒pSDJ2009-52F的序列，该质粒带有VAG的典型库和IS 26侧翼的复杂抗性基因座，这是一种在动员质粒和基因岛上的抗生素抗性基因中起重要作用的插入元件。我们在20年的时间范围内，从与pSDJ2009-52F共享> 80％序列同一性的公共数据库中恢复了完整的ColV样质粒序列。先前，我们注意到pSDJ2009-52F在1级整合子中具有独特的遗传标记，这可能是由IS 26的作用所引起的。在这里，我们显示与pSDJ2009-52F密切相关的大多数ColV样质粒也带有相同的标记。我们的研究提供了对这些带有特征的质粒以及它们如何在大肠杆菌之间进行运输的流动遗传元件的见解。 较大地理距离上的序列类型。