Backgrounds/Aims

Pancreatic cancer is a digestive system tumour disease with a notably poor prognosis and a 5-year survival rate of less than 10 %. In recent years, peptide drugs have shown great clinical value in antitumour applications. We aim to identify differentially expressed peptides by using peptidomics techniques to explore the mechanisms involved in the development and pathology of pancreatic cancer.

Methods

We performed peptidomic analysis of pancreatic cancer and paired paracancerous tissues by using ITRAQ labelling technology and conducted in-depth bioinformatics analysis and functional studies on differentially expressed peptides.

Results

A total of 2,881 peptides were identified, of which 133 were differentially expressed (116 were upregulated and 17 were downregulated). By using GO analysis, the differentially expressed peptides were found to be closely related to the tumour microenvironment and extracellular matrix. KEGG enrichment analysis revealed that precursor proteins were closely related to the T2DM and RAS signalling pathways. The endogenous peptide P1DG can significantly inhibit the proliferation, migration and invasion of pancreatic cancer cells.

Conclusion

P1DG and its precursor GAPDH may be closely related to the proliferation, migration and invasion of pancreatic cancer. Peptidomics can aid in understanding the pathogenesis of pancreatic cancer more comprehensively.

中文翻译：

---

比较性的肽组分析揭示了肽在胰腺癌病理中的关键作用。

背景/目标

胰腺癌是一种消化系统肿瘤疾病，预后特别差，5年生存率不到10％。近年来，肽类药物在抗肿瘤应用中已显示出巨大的临床价值。我们旨在通过使用肽组学技术来鉴定差异表达的肽，以探索参与胰腺癌的发展和病理学的机制。

方法

我们使用ITRAQ标记技术对胰腺癌和癌旁组织进行了肽组分析，并对差异表达的肽段进行了深入的生物信息学分析和功能研究。

结果

总共鉴定出2881个肽，其中133个差异表达（116个上调，而17个下调）。通过GO分析，发现差异表达的肽与肿瘤微环境和细胞外基质密切相关。KEGG富集分析表明，前体蛋白与T2DM和RAS信号通路密切相关。内源肽P1DG可以显着抑制胰腺癌细胞的增殖，迁移和侵袭。

结论

P1DG及其前体GAPDH可能与胰腺癌的增殖，迁移和侵袭密切相关。肽组学可以帮助更全面地了解胰腺癌的发病机理。