当前位置: X-MOL 学术Appl. Spectrosc. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
EXPRESS: Raman Spectroscopy: An Exploratory Study to Identify Post-Radiation Cell Survival
Applied Spectroscopy ( IF 2.2 ) Pub Date : 2020-05-01 , DOI: 10.1177/0003702820908352
Kshama Pansare 1 , Saurav Raj Singh 1 , Venkatavaradhan Chakravarthy 1 , Neha Gupta 1 , Arti Hole 1 , Poonam Gera 1 , Rajiv Sarin 1, 2 , Chilakapati Murali Krishna 1, 2
Affiliation  

Resistance to radiotherapy has been an impediment in the treatment of cancer, and the inability to detect it at an early stage further exacerbates the prognosis. We have assessed the feasibility of Raman spectroscopy as a rapid assay for predicting radiosensitivity of cancer cells in comparison to the conventional biological assays. Cell lines derived from breast adenocarcinoma (MCF7), gingivobuccal squamous cell carcinoma (ITOC-03), and human embryonic kidney (HEK293) were subjected to varying doses of ionizing radiation. Cell viability of irradiated cells was assessed at different time points using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Raman spectroscopy, and colony-forming capability was evaluated by clonogenic assay. Radiosensitivity observed using MTT assay was limited by the finding of similar cell viability in all the three cell lines 24 h post-irradiation. However, cell survival assessed using clonogenic assay and principal component linear discriminant analysis (PC-LDA) classification of Raman spectra showed correlating patterns. Irradiated cells showed loss of nucleic acid features and enhancement of 750 cm−1 peak probably attributing to resonance Raman band of cytochromes in all three cell lines. PC-LDA analysis affirmed MCF7 to be a radioresistant cell line as compared to ITOC-03 and HEK293 to be the most radiosensitive cell line. Raman spectroscopy is shown to be a rapid and alternative assay for identification of radiosensitivity as compared to the gold standard clonogenic assay.

中文翻译:

EXPRESS:拉曼光谱:一项确定辐射后细胞存活率的探索性研究

对放疗的抵抗一直是癌症治疗的障碍,无法在早期发现它进一步加剧了预后。我们已经评估了拉曼光谱作为一种与传统生物测定相比预测癌细胞放射敏感性的快速测定的可行性。来自乳腺腺癌 (MCF7)、龈颊鳞状细胞癌 (ITOC-03) 和人胚胎肾 (HEK293) 的细胞系受到不同剂量的电离辐射。使用MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑)测定和拉曼光谱在不同时间点评估照射细胞的细胞活力,并通过克隆形成测定评估集落形成能力. 使用 MTT 测定观察到的放射敏感性受到在辐射后 24 小时所有三种细胞系中相似细胞活力的发现的限制。然而,使用拉曼光谱的克隆形成测定和主成分线性判别分析 (PC-LDA) 分类评估的细胞存活显示相关模式。受照射的细胞表现出核酸特征的丧失和 750 cm-1 峰的增强,这可能归因于所有三种细胞系中细胞色素的共振拉曼带。PC-LDA 分析证实 MCF7 是抗辐射细胞系,而 ITOC-03 和 HEK293 是辐射最敏感的细胞系。与金标准克隆形成分析相比,拉曼光谱被证明是一种快速和替代的放射敏感性鉴定方法。使用克隆形成试验和主成分线性判别分析 (PC-LDA) 对拉曼光谱进行分类评估的细胞存活率显示出相关模式。受照射的细胞表现出核酸特征的丧失和 750 cm-1 峰的增强,这可能归因于所有三种细胞系中细胞色素的共振拉曼带。PC-LDA 分析证实 MCF7 是抗辐射细胞系,而 ITOC-03 和 HEK293 是辐射最敏感的细胞系。与金标准克隆形成分析相比,拉曼光谱被证明是一种快速和替代的放射敏感性鉴定方法。使用克隆形成试验和主成分线性判别分析 (PC-LDA) 对拉曼光谱进行分类评估的细胞存活率显示出相关模式。受照射的细胞表现出核酸特征的丧失和 750 cm-1 峰的增强,这可能归因于所有三种细胞系中细胞色素的共振拉曼带。PC-LDA 分析证实 MCF7 是抗辐射细胞系,而 ITOC-03 和 HEK293 是辐射最敏感的细胞系。与金标准克隆形成分析相比,拉曼光谱被证明是一种快速和替代的放射敏感性鉴定方法。受照射的细胞表现出核酸特征的丧失和 750 cm-1 峰的增强,这可能归因于所有三种细胞系中细胞色素的共振拉曼带。PC-LDA 分析证实 MCF7 是抗辐射细胞系,而 ITOC-03 和 HEK293 是辐射最敏感的细胞系。与金标准克隆形成分析相比,拉曼光谱被证明是一种快速和替代的放射敏感性鉴定方法。受照射的细胞显示出核酸特征的丧失和 750 cm-1 峰的增强,这可能归因于所有三种细胞系中细胞色素的共振拉曼带。PC-LDA 分析证实 MCF7 是抗辐射细胞系,而 ITOC-03 和 HEK293 是辐射最敏感的细胞系。与金标准克隆形成分析相比,拉曼光谱被证明是一种快速和替代的放射敏感性鉴定方法。
更新日期:2020-05-01
down
wechat
bug