In this study, loop-mediated isothermal amplification (LAMP) was used to establish a rapid, specific, and visual detection method for duck hepatitis B virus (DHBV). The design and synthesis of 4 specific LAMP primers were based on the conserved gene region of the DHBV genome, and the optimum temperature and time of the LAMP reaction were 63°C and 50 min, respectively. The LAMP assay was confirmed to be specific for DHBV detection and had the same sensitivity as the quantitative PCR assay. A visual detection method for rapid determination of results was developed using a color indicator containing phenol red and cresol red. A color change was produced based on a pH change in the reaction system, indicating a positive reaction. For the detection of samples from ducks and geese, the LAMP method has the advantages of simplicity, high sensitivity and specificity, good visibility, and low cost. Moreover, it is more practical and convenient than PCR-related assays for the clinical detection of DHBV.

中文翻译：

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使用环介导的等温扩增简单直观地检测鸭和鹅中的鸭乙肝病毒。

在这项研究中，环介导的等温扩增（LAMP）用于建立鸭乙型肝炎病毒（DHBV）的快速，特异性和视觉检测方法。根据DHBV基因组的保守基因区域设计和合成了4种特异性LAMP引物，LAMP反应的最佳温度和时间分别为63°C和50 min。LAMP测定被证实对DHBV检测具有特异性，并具有与定量PCR测定相同的灵敏度。使用含有酚红和甲酚红的颜色指示剂开发了一种视觉检测方法，用于快速确定结果。基于反应体系中pH的变化产生颜色变化，表明反应是阳性的。对于检测鸭和鹅的样品，LAMP方法具有简单，易用，高灵敏度和特异性，良好的可见性和低成本。而且，对于DHBV的临床检测，它比PCR相关的检测方法更加实用和方便。