Adult myoblasts, satellite cells, will proliferate, and differentiate into myotubes in vitro. However, changes in environmental and nutritional conditions will result in the satellite cells differentiating into adipocyte-like cells synthesizing lipids. Prior research has shown that levels of N-glycosylation and heparan sulfate can promote or prevent the adipogenic conversion of myogenic satellite cells. Syndecan-4, an N-glycosylated heparan sulfate proteoglycan, has been shown to play key roles in satellite cell proliferation and migration. The objective of the current study was to determine if syndecan-4, and syndecan-4 N-glycosylation and heparan sulfate chain levels altered the conversion of satellite cells to an adipogenic cell fate and if growth selection affected the response of the satellite cells. Over-expression of syndecan-4, syndecan-4 without N-glycosylated chains but with its heparan sulfate chains attached, syndecan-4 without heparan sulfate chains with its N-glycosylation chains, and syndecan-4 without N-glycosylation and heparan sulfate chains was measured for lipid accumulation in pectoralis major muscle satellite cells isolated from the Randombred Control line 2 (RBC2) and 16 wk body weight (F line) turkeys. The F line was selected from the RBC2 line for only 16 wk body weight. Results from this study demonstrated that wild type levels of syndecan-4 and its covalently attached N-glycosylation chains play a key role in regulating the conversion of pectoralis major muscle satellite cells to an adipogenic lineage while selection for body weight was not a major contributing factor in this conversion.

中文翻译：

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研究报告：体重选择对 Syndecan-4 及其共价连接的 N-糖基化链对火鸡生肌卫星细胞脂肪形成转化的影响。


成体成肌细胞（卫星细胞）将在体外增殖并分化为肌管。然而，环境和营养条件的变化将导致卫星细胞分化为合成脂质的脂肪细胞样细胞。先前的研究表明，N-糖基化和硫酸乙酰肝素的水平可以促进或阻止肌源性卫星细胞的脂肪形成转化。 Syndecan-4 是一种 N-糖基化硫酸乙酰肝素蛋白聚糖，已被证明在卫星细胞增殖和迁移中发挥关键作用。当前研究的目的是确定syndecan-4、syndecan-4 N-糖基化和硫酸乙酰肝素链水平是否改变卫星细胞向脂肪形成细胞命运的转变，以及生长选择是否影响卫星细胞的反应。 syndecan-4、不带 N-糖基化链但附有硫酸乙酰肝素链的 syndecan-4、不带硫酸乙酰肝素链但具有 N-糖基化链的 syndecan-4 以及不带 N-糖基化和硫酸乙酰肝素链的 syndecan-4 过表达测量从随机饲养对照系 2 (RBC2) 和 16 周体重（F 系）火鸡分离的胸大肌卫星细胞中的脂质积累。 F系选自仅16周体重的RBC2系。这项研究的结果表明，野生型水平的 syndecan-4 及其共价连接的 N-糖基化链在调节胸大肌卫星细胞向脂肪形成谱系的转化中发挥着关键作用，而体重选择并不是主要影响因素在这个转换中。